普通小麦-百萨偃麦草(Thinopyrum bessarabicum)二体异附加系的选育与鉴定  

Development and Identification of a Set of Triticum aestivum-Thinopyrum bessarabicum Disomic Alien Addition Lines

作　　者：庄丽芳[1] 亓增军[1] 英加[1] 陈佩度[1] 刘大钧[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室,细胞遗传研究所南京210095

出　　处：《Acta Genetica Sinica》2003年第10期919-925,共7页

基　　金：麦氏基金项目 (CCRP);国家自然科学基金项目 (3 0 2 0 0 175 )资助~~

摘　　要：为转移与利用百萨偃麦草耐盐、抗病等优良基因 ,用普通小麦中国春 百萨偃麦草双倍体与中国春杂交 ,通过染色体C 分带、分子原位杂交并结合减数分裂中期Ⅰ的染色体配对分析 ,从回交后代中选育出一套小麦 百萨偃麦草二体异附加系。对这套异附加系进行的鉴定与分析表明 ,各附加系除添加了一对百萨偃麦草染色体外 ,小麦的 2 1对染色体未见明显变化。各附加系所添加的百萨偃麦草染色体在减数分裂中期Ⅰ配对基本正常 ,仅有少量单价体 ,其自交后代中外源染色体亦能正常传递。这说明所培育的这套二体异附加系在细胞学上已相对稳定 ,暂分别编号为DAJ1 、DAJ2 、DAJ3、DAJ4 、DAJ5、DAJ6 和DAJ7。In order to transfer the genes for salt tolerance a nd disease resistance from Thinopyrum bessarabicum into wheat,the hybrid pr ogenies between T.aestivum cv.Chinese Spring and T.aestivum cv.Chinese Spring-amphiploid Th.bessarabicum were screened.A set of T.aestiv um-Th.bessarabicum disomic addition lines was developed with the assistance o f mitotic chromosome C-banding and genomic in situ hybridization (GISH),as well as GISH on meiotic MⅠ chromosome preparations.The result s indicated that a ll the wheat chromosomes in the lines remained unchanged karyotypically,while th e added Th.bessarabicum chromosomes paired regularly in meiosis.The develope d disomic addition lines were designated temporarily as DAJ 1,DAJ 2,DAJ 3, DAJ 4,DAJ 5,DAJ 6 and DAJ 7 respectively.Determination of the homoeologous groups of the added Th.bessarabicum chromosomes and localization of the gen es for salt tolerance and disease resistance are undergoing.

关 键 词：普通小麦 百萨偃麦草 二体异附加系 

分 类 号：S512.1[农业科学—作物学]