高血压患者血管紧张素原基因调控及与细胞因子关系的研究  

Studies of the Assoiation Between Angiotens inogen Gene Regulation and Cytokines in Essential Hypertension

作　　者：王爱玲[1] 余元勋[2] 徐岩[1] 刘萍[2] 程自平[1] 张立[2] 吴继雄[1] 孙国梅[2] 陈森[1] 

机构地区：[1]安徽医科大学第一附属医院心内科,合肥230022 [2]安徽省优生优育遗传医学中心,合肥230031

出　　处：《Acta Genetica Sinica》2003年第10期978-982,共5页

基　　金：安徽省教育厅自然科学基金项目 (2 0 0 2kj14 0 );安徽省重点科研项目 (2 0 0 3 0 2 10 0 7)~~

摘　　要：在高血压患者中研究血管紧张素原 (AGT)基因M2 35T多态性、等位基因分离及与细胞因子的关系。采用聚合酶链反应结合内切酶消化进行AGT基因型分析。用双抗体夹心ELISA酶联法测定细胞因子 (IL 1、IL 6、TNF)。分析高血压病组和对照组的血管紧张素原 (AGT)基因 2 35位氨基酸残基的基因型及等位基因频率 ,并分析细胞因子对AGT基因M2 35T变异的影响。结果表明 :高血压组中AGT 2 35为TT型 38例 (5 5 88% ) ,TM型 2 4例(35 2 9% ) ,MM型 6例 (8 82 % ) ,其T型和M型等位基因频率分别为 73 5 3%和 2 6 4 7%。对照组中TT型 2 8例(47 4 6 % ) ,TM型 2 5例 (42 37% ) ,MM型 6例 (10 17% ) ,T型和M型等位基因频率分别为 6 8 6 5 %和 31 35 %。在AGT 2 35T等位基因型组中 ,高血压病组IL 1、IL 6、TNF浓度显著高于对照组。在AGT 2 35M等位基因型组中 ,TNF浓度高于对照组。无论大于 6 0岁或小于 6 0岁的高血压病与相应年龄对照组比较 ,IL 1、IL 6、TNF浓度均显著升高。而在高血压病或对照组内 ,大于 6 0岁组与小于 6 0岁组之间比较 ,IL 1、IL 6、TNF浓度均无显著性差异。说明IL 1、IL 6、TNF升高与疾病有关而与年龄无关。研究结论 :高血压病AGT 2 35TT型和T等位基因频率高于健康对照。AGT 2 35TT型和T等位基因频率?This study is to explore the association between M2 35T allele polymorphism of angiotensinogen (AGT) gene and cyto kines using essential hypertension probands research method.In hypertensives and controls,polymerase chain reaction combined with restriction endonuclease diges tion was used to detect the target genotype variation,and enzyme-lined immunoso rban t assay (ELISA) was used to detect the cytokine concentrations (IL-1,IL-6,TNF) .The results showed that in hypertensives AGT gene,TT genotype was 55.8 8%,MT 35.29% and MM 8.82%.The ratio of T/M allele frequency was 0 .735/0.265.In controls AGT gene,TT genotype was 47.46%,MT 42.37% and MM 10.17%.The ratio of T/M allele frequency was 0.686/0.314.AG T gene 235 T allele frequency in hypertensives was slightly higher than those in controls.Furthermore AGT gene 2 35 TT genotype and T allele frequency in middle and high grade of hypertensives were significantly higher than those in mild grade.In subjects of AGT 235 T allele group,the concentrations of I L-1,IL-6 and TNF in hypertensives were significantly higher than those in co nt rols.In subjects of AGT gene 235 M allele freque ncy,the concentrations of IL-1 and IL-6 in hypertensives were no significant t han those in controls.No matter in groups more than 60 years old or less than 60 years old,the concentrations of IL-1,IL-6 and TNF in hypertensives were highe r than those in controls.No matter in hypertensives or controls,there were no di fferences in concentrations of IL-1、IL-6 and TNF when comparing groups more t han 60 years old with groups less than 60 years old.The study indicated that A GT gene TT genotype and AGT gene 235 T all ele frequency may be an important risk factor for hypertension.The high frequenc y of AGT gene 235 T allele and the high concentr ations of IL-1、IL-6 and TNF in hypertensives may cause hypertension developin g.It is also suggested the cytokines may effect the transcription and expression of AGT gene 235 TT genotype in hypertension.The concentrations of IL-1 、IL-6 and TNF ha

关 键 词：原发性高血压 AGT基因 细胞因子 

分 类 号：R544.1[医药卫生—心血管疾病]