异种大鼠神经干细胞脑内移植未导致明显的免疫排斥反应  

Immunal rejection not occurred after intracerebral transplantation of heterogenous neural stem cells in rats

作　　者：程小燕[1] 王红云[1] 安沂华[1] 

机构地区：[1]北京市神经外科研究所,北京市100050

出　　处：《中国康复理论与实践》2003年第9期541-542,共2页Chinese Journal of Rehabilitation Theory and Practice

基　　金：中国博士后基金;北京市科技新星计划 (No.H0 2 0 82 1 360 1 30 )资助项目

摘　　要：目的探讨异种大鼠神经干细胞脑内移植是否会导致明显的免疫排斥反应。方法制作SD大鼠大脑中动脉夹闭致脑缺血模型 ,将体外培养的Wistar大鼠神经干细胞移植到脑缺血同侧或对侧的纹状体区 ,在移植后的不同时间杀死大鼠 ,研究脑内单核吞噬细胞的侵润、脾脏淋巴滤泡增殖以及脑内干细胞存活和迁移的情况。结果移植后的干细胞在脑内大量存活并向损伤区域迁移 ;与未接受干细胞移植的对照组相比较 ,干细胞移植组大鼠并未发生明显的脑内单核吞噬细胞侵润和脾脏淋巴滤泡的大量增殖。结论异种神经干细胞脑内移植未导致明显的免疫排斥反应。ObjectiveTo investigate whether intracerebral transplantation of heterogenous neural stem cells(NSCs) may cause immunal rejection or not.MethodsNSCs derived from the brain of embryonic 16-day Wistar rat were cultured in vitro. Cerebral ischemic SD rat models were made by middle cerebral artery occlusion and NSCs were transplanted into the ipsilateral or contralateral caudate nucleus 4 days later. Rats were sacrificed at different time points after transplantation, the reaction and proliferation of mononuclear phagocytic system(MPS) and splenic follicles as well as the survival and migration of NSCs were checked.ResultsNumerous NSCs survived and migrated toward the lesion in vivo. Comparing with control group that did not receive NSCs transplantation, there was no significant infiltration of mononuclear phagocytes or proliferation of splenic follicles in NSCs transplanted rats.ConclusionsIntracerebral transplantation of heterogenous neural stem cells did not cause remarkable immunal rejection. [

关 键 词：干细胞 移植 脑梗死 免疫排斥 

分 类 号：R318.5[医药卫生—生物医学工程] R651[医药卫生—基础医学]