角蛋白19在Ⅲ度烧伤创面修复过程中的表达及意义  

Expression of Cytokeratin 19 in Different Live Tissues From Victims With Third-degree Burns in Repair Process

作　　者：周鹏翔[1] 罗成群[1] 谷永红[2] 贺全勇[1] 李高峰[1] 彭长缨[2] 彭浩[1] 

机构地区：[1]中南大学湘雅三医院烧伤科,湖南长沙410013 [2]中南大学湘雅三医院病理科

出　　处：《中国医师杂志》2003年第9期1180-1182,共3页Journal of Chinese Physician

摘　　要：目的　通过对Ⅲ度烧伤创面原位再生修复过程中不同活组织角蛋白 19(K19)表达特征的研究 ,探索Ⅲ度烧伤创面原位再生修复的可能机制。方法　选择有Ⅲ度烧伤创面的青年患者 10例 ,在原位再生修复过程中的不同时期分别切取活组织 ,应用过氧化酶标记的链霉卵白素 (SP)免疫组化法 ,以K19单抗检测表皮干细胞的分布和形态特征 ;同时取供皮区的正常皮肤以及慢性溃疡组织作对照。结果　⑴正常皮肤组织只有表皮基底细胞层和附属器的部分细胞表达K19;⑵伤后 3～ 6d ,10例患者的痂下活组织均未发现K19表达阳性细胞 ;⑶肉芽样组织有部分细胞表达K19;⑷早期再生组织检测结果示 :在再生表皮的中部 ,即位于基底膜与颗粒层之间有大量的K19表达阳性细胞 ,且分布广泛而有规律 ;⑸晚期再生组织检测结果显示 :与正常皮肤类似 ;⑹入院前慢性皮肤溃疡组织中无K19表达阳性细胞。结论　Ⅲ度烧伤创面经烧伤湿性医疗技术加rhEGF治疗后K19表达阳性细胞可以从无到有产生并不断增加 。Objective To study the expression characteristics of cytokeratin 19(CK19) in different live tissues from victims with third-degree burns to explore the possible mechanisms of tissue reparation and regeneration in orthophoria.Methods 10 young victims with third-degree burns were included in this study.Alive tissues at different regeneration stages were studied by immunohistochemistry with streptavidin-biotin-peroxidase method using specific anti-CK19 monoclonal antibody. Only cytoplasm expression was considered as specific.Normal skin tissue and chronic skin ulcer were used as controls.The study was focused on the distribution and morphological features of CK19-positive cells.Results ⑴In normal skin ,CK19-positive cells were seen in basialis layer of epiderm and cutaneous appendages.⑵CK19-positive cells were not found in subcutaneous tissue at 3～6 days after the injury,but they were seen in granulation tissues.⑶A lot of CK19-positive cells were detected in early regeneration tissues.⑷In late regeneration tissues,CK19-positive cells were detected in basialis layer of epiderm and cutaneous appendages.⑸Advanced regenerationed epidermal tissue was similar to that of normal.⑹No CK19-positive cells were observed in all of 6 cases with chronic skin ulcer.Conclusion It is possible that victims with third-degree burns could be healed by tissue regeneration orthophoria.

关 键 词：烧伤 角蛋白 表皮干细胞 

分 类 号：R644[医药卫生—外科学]