水稻对低剂量UV-B辐射胁迫的分子应答研究  

Molecular responses of rice (Oryza sativa L.) to the stress of lowly enhanced UV-B radiation

作　　者：曾建敏[1] 林文雄[1] 梁康迳[1] 陈芳育[1] 郭玉春[1] 唐莉娜[1] 何华勤[1] 王经源[1] 梁义元[1] 

机构地区：[1]福建农林大学农业生态研究所,福州350002

出　　处：《应用生态学报》2003年第6期941-944,共4页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金项目 (3 9970 44 1);教育部优秀青年教师基金(2 0 0 0 11);福建省自然科学基金资助项目(B0 2 10 0 14 )

摘　　要：采用mRNA差异显示技术 ,分析了水稻幼苗受低剂量UV B辐射的mRNA表达丰度差异 ,获得了 18个差异片段 ,其中 15个差异片段在表达水平上有差别 ,1条为被抑制表达 ,而UV B诱导特异表达的为 2条 .将这 2条片段进一步回收、重扩增发现确为特异带型 .对其中的一个差异片段RUVB2进行序列测定和国际互联网查询结果表明 ,该序列位于Oryzasativa第 10染色体的OSJNBb0 0 91N2 1BAC上 (登录号 :AC0 9112 2 ) ,且该cDNA片段与水稻的 3个EST序列有 95 %以上的同源性 。The approach of mRNA differential display (DD) was used to analyze the gene differential expression of rice seedlings subjected to lowly enhanced Ultraviolet B radiation. The results showed that of the eighteen differential fragments, fifteen ones were different in expressional levels, another one was depressed, and the other two, named as RUVB1 and RUVB2 respectively,which could be recovered and re amplified, were specifically induced. Sequence analysis for RUVB2 through Internet BLAST searching indicated that it was more than 95% identities with three rice ESTs, but very low similarity to others in the GenBank, and was mapped on the OSJNBb0091N21BAC(GenBank accession No.:AC091122) in Oryza sativa L.

关 键 词：分子应答 MRNA差异显示 水稻 UV—B 

分 类 号：S511[农业科学—作物学]