基因重组(酵母)乙肝疫苗对HBV转基因小鼠细胞免疫调节作用  

Cellular Immune Regulation Effect of Recombinant Hepatitis B Vaccine on HBV Transgenic Mice

作　　者：易学瑞[1] 曾滢 祖萍[1] 朱征宇 刘光泽[1] 甘建辉 袁有成[1] 汪恩浩 张宜俊[1] 

机构地区：[1]中国人民解放军第四五八医院全军肝病中心,广州510602 [2]深圳市南山区科技园深圳康泰生物制品股份有限公司,深圳518057

出　　处：《上海实验动物科学》2003年第3期151-153,156,共4页Shanghai Laboratory Animal Science

摘　　要：为研究基因重组乙肝疫苗对HBV转基因小鼠的免疫调节作用,取经HBsAg免疫的小鼠脾淋巴细胞,制成5×106细胞悬液,分两份,一份经无佐剂HBsAg体外刺激48h后,取上清检测细胞因子;另一份细胞于培养56h后加入3H-TdR,继续培养16h,测定cpm值。结果表明,转基因鼠经多次免疫8个月后,其脾细胞产生的Th1类细胞因子(IL-2、IFN-γ)及T细胞增殖水平均较单次免疫一周后有显著性提高。而采用不同剂量HBsAg按常规免疫时并不能提高IL-2的水平。结果提示,反复大剂量的HBsAg可以打破HBV转基因小鼠的免疫耐受,上调其细胞免疫功能,这为慢性乙肝的临床治疗提供了实验根据。To study the celluar immune responses of HBV transgenic (Tg) mice induced by genetic recombinant Hepatitis B vaccine, the HBV transgenic mice were immunized by Hepatitis B vaccine,and killed at different period. The spleen were removed, and lymphocyte were separated and divided into two parts. One were cultured for 48h with HBsAg and assayed for IL2 and IFNγand the other part were labelled with 3HTdR after cultured for 56 h, and cultured for another 16h.The culture were harvested and the cpm was determined by using a liquid scintillation counter. The result showed that the Th1 cytokine production and lymphocyte proliferation of HBV Tg mice after repeated immunization for 8 months were much higher than those of immunized one time for 1 week.But after immunized as normal style with different doses,the IL2 level were not different to one time immunization. The results suggested that the high dose with repeated immunization might broken the immunized tolerance of HBV Tg mice and upregulate celluar responses.

关 键 词：乙型肝炎 乙肝疫苗 转基因小鼠 免疫耐受 Thl细胞因子 T细胞增殖 

分 类 号：R512.62[医药卫生—内科学]