费氏中华根瘤菌共生质粒扩增对结瘤因子组分和共生固氮能力的影响  

Effect of Sinorhizobium fredii YC4 symbiotic plasmid amplification on nod factors and symbiotic N-fixation.

作　　者：缪礼鸿[1] 周俊初[1] 

机构地区：[1]华中农业大学教育部微生物重点实验室,武汉430070

出　　处：《应用生态学报》2003年第9期1517-1520,共4页Chinese Journal of Applied Ecology

基　　金：欧盟INCO-DC国际合作项目(2001AA214021);国家基础研究发展规划资助项目(001CB1089)

摘　　要：费氏中华根瘤菌(Sinorhizobium fredii)YC4能在大豆(Glycine max)和野大豆(G.soia)上形成正常固氮的根瘤。人工培养条件下用^(14)C标记的薄层层析(TLC)法检测根瘤菌产生的结瘤因子(LCOs)的结果表明,与其它4株费氏中华根瘤菌相比,YC4产生的LCOs含有较多的疏水性基因。从YC4菌株中分离到1株共生质粒发生了扩增的自发突变株YSC3,其产生的LCOs中含有较野生型菌株多的1个疏水性组分,28℃培养条件下产生的LCOs量亦较YC4显著增加。结瘤试验结果表明,YSC3菌株只能在大豆和野大豆上形成无效的根瘤。Sinorhizobium fredii YC4 can form nitrogen-fixing nodules on soybean (Glycine max) and wild soybean (G. soja).It can produce unique lipochitooligosaccharide nod factors (LCOs), in comparison with the other four strains of S. fredii.The constitution of LCOs produced by YC4 contained more hydrophobic substitutions detected by Thin-layer chromatography (TLC) of 14C-labeled nod factors. A spontaneous mutant termed YSC3 amplified in the symbiotic plasmid was isolated from YC4, which can produce more amount of LCOs than its parental strain at 28 癈 , and showed a difference in the construction of LCOs. Nodulation test indicated that YSC3 only formed ineffective nodules on soybean G. soja .

关 键 词：费氏中华根瘤菌 共生质粒 结瘤因子 共生固氮 

分 类 号：S144.5[农业科学—肥料学]