吖啶橙-SDS-核酸荧光体系的研究及分析应用  

Studies of Interaction of DNA and AO with SDS by Fluorescent Analysis and Its Analysis Application

作　　者：陈艳晶[1] 卢圣欣[1] 

机构地区：[1]济南大学化学化工学院,山东济南250022

出　　处：《济南大学学报（自然科学版）》2003年第3期265-267,共3页Journal of University of Jinan(Science and Technology)

摘　　要：在pH =5 .89的六次甲基四胺 -HCl缓冲溶液中 ,DNA可使吖啶橙 (AO)的荧光强度增强 ,在一定浓度范围内 ,体系的荧光强度变化与DNA浓度呈线性关系 ,可用于DNA的定量测定。体系的荧光强度在加入阴离子表面活性剂十二烷基硫酸钠 (SDS)后有很大增强。研究了pH值及缓冲溶液种类、吖啶橙用量、表面活性剂种类及用量等对体系荧光强度的影响。结果表明 ,在最佳实验条件下 ,DNA浓度的线性范围分别为 0～ 2 .0 μg/mL、2 .0～ 10 .0 μg/mL ,检出限为 7.18ng/mL ,相对标准偏差为 2 .0 %。该方法线性范围较宽、灵敏度高、反应速度快、稳定时间长 ,是用染料定量分析DNA的一种无毒、高效、简便的新方法。Acridine orange, a fluorescent dye, was used as a biological fluorescent probe for the determination of DNA. The calibration cures for the determination of DNA were linear over the range of 0-2.0?μg/mL and 2.0-10.0?μg/mL.The detection limit is 7.18?ng/mL.The relative standard deviation was within 2.0% in the middle of linear range. Interference concerning some interesting coexisting substances with the determination of DNA was also examined. The significant features of this method are its high sensitivity and rapidity of reaction, wide range of linearity, high accuracy and selectivity.

关 键 词：荧光分析 吖啶橙 核酸 

分 类 号：O657.3[理学—分析化学]