高压氧对精索静脉曲张家兔睾丸组织学影响的实验研究  

Effects of Hyperbaric Oxygenation on Rabbits' Testes with Varicocele

作　　者：郭永连 周四维[2] 张小平 

机构地区：[1]襄樊市第一人民医院泌尿外科,湖北襄樊441000 [2]华中科技大学同济医学院附属同济医院泌尿外科,湖北武汉430030

出　　处：《中华男科学杂志》2003年第6期443-446,450,共5页National Journal of Andrology

摘　　要：目的 :通过研究高压氧 (HBO)对精索静脉曲张 (VC)家兔睾丸组织形态和功能的影响 ,进一步探讨VC导致男性不育的发病机理。　方法 :健康成熟雄兔随机分为假手术对照组、VC模型组及高压氧干预的VC模型组 3组 ,每组 8只。部分结扎家兔左腰睾丸干静脉建立VC模型 ,术后用HBO对动物模型进行干预 ,对模型的精液参数 ,睾丸的重量、体积 ,睾丸组织形态以及精曲小管平均直径 (MTDs)、精曲小管生育力指数 (TFI)、Sertoli细胞指标 (SI)进行研究。　结果 :HBO干预动物的精液质量明显改善 ,睾丸重量、体积明显增加 ,与VC模型组比较MTDs差异具有极显著性 (P <0 .0 0 0 1) ,睾丸组织形态基本正常。　结论 :①VC可导致睾丸病理损害和生精功能障碍 ;②慢性缺血、缺氧和微循环障碍可能是VC导致睾丸病理损害、生精功能障碍的首要原因和中心环节 ;③HBO可有效减轻甚至消除VC睾丸组织慢性缺血、低氧状态 ,改善其微循环灌注 ,保护VC睾丸的结构和功能。Objective: To study the mechanism of male infertility caused by varicocele by evaluating the effects of hyperbaric oxygenation (HBO) therapy on the testicular tissue morphology and function of rabbit model with varicocele(VC). Methods: Twenty-four mature male rabbits were randomly divided into three groups: pseudo-operation, VC model and VC model administered by HBO. Experimental varicocele was induced by partial ligation of the left 'lumbotesticular' trunk vein in rabbits. HBO was administered to one of the two groups of VC model rabbits after the operation. Weight and volume of both testes, parameters of seminal fluid, histological changes of testicular tissues, MTDs, TFI, and Sertoli cell index (SI) of seminiferous tubules were studied. Results: The average weight and volume of the left testes significantly increased in the rabbits treated by HBO. The semen quality was improved, and MTDs increased significantly compared with VC group(P< 0.000 1). The testicular tissue morphology became nearly normal in VC+HBO group. Conclusions: ①Both the structure and spermatogenetic function of testes can be damaged by the presence of varicocele; ②Chronic ischemia, anoxia and microcircular dysfunction may be the key process and essential factor that make varicocele contributive to testicular damage and spermatogenetic dysfunction; ③HBO can effectively alleviate, even eliminate, chronic ischemia, anoxia and microcircular dysfunction in testicular tissues with varicocele, and thus protect the structure and functions of testes.

关 键 词：精索静脉曲张 睾丸 高压氧 不育症 家兔 

分 类 号：R697.23[医药卫生—泌尿科学]