一种人外周血NK细胞高效扩增的方法  

A Study of the method of Expansion of NK Cells from Human Peripheral Blood

作　　者：黄朝晖[1] 王丰[1] 华东[1] 刘志辉[1] 李莉华[1] 任金冬[1] 

机构地区：[1]苏州大学附属第四医院肿瘤研究所中心实验室,江苏无锡214062

出　　处：《江南大学学报（自然科学版）》2003年第3期324-326,共3页Joural of Jiangnan University (Natural Science Edition)　

基　　金：无锡市卫生局"十五"重大科研项目(2002~2003)资助课题

摘　　要：目的 探索从人PBMC中高效扩增CD3^- CD56^+NK细胞的方法。方法 使用干细胞生长培养基,在抗CD3单抗和IL-2的作用下,从健康成人PBMC中诱导扩增CD3^- CD56^+NK细胞,并用流式细胞仪检测扩增细胞的免疫表型。结果 PBMC经抗CD3单抗、IL-2联合作用获得大量增殖,在14d时扩增54.3±5.5倍,含有(52.4±7.9)％CD3^- CD56^+NK细胞。结论 可使用干细胞生长培养基,在抗CD3单抗和IL-2协同刺激下大量扩增NK细胞,为应用NK细胞进行肿瘤过继免疫治疗提供了一种简单有效的扩增NK细胞的方法。OBJECTIVE: To study the method of expansion of CD3-、CD56 + NK cells from peripheral blood mononuclear cells(PBMCs). METHOD: PBMCs were cultured in stem cell growth medium (SCGM) supplemented with monoclonal anti-CD3 antibodies and IL-2 with different concentrations; The immune phenotypes of expanded cells were analyzed by Flow cytometry (ECM). RESULT: After being activated by monoclonal anti-CD3 antibodies and IL-2, PBMCs got the best expansion and expanded to 56. 2±5. 2 folds in 14th day after incubation, and contained (52.4±7.9)% CD3-、CD56+ NK cells. CONCLUSION: NK cells could be expanded from PBMCs using SCGM with the synergistic induction by monoclonal anti-CD3 antibodies and IL-2. This work provided a simple and effective method of expanding and enriching human NK cells for the adoptive immunotherapy of malignant tumors

关 键 词：NK细胞 抗CD3单抗 白介素—Ⅱ 过继免疫治疗 

分 类 号：R392-33[医药卫生—免疫学]