川芎嗪和剪切率对悬浮血管内皮细胞凋亡的影响  

INFLUENCES OF LIGUSTRAZINE AND SHEAR RATE ON THE APOPTOSIS OF SUSPENDING ENDOTHELIAL CELL

作　　者：欧阳志钢[1] 韩东[2] 李文[1] 廖福龙[1] 

机构地区：[1]中国中医研究院中药研究所,北京100700 [2]清华大学物理系

出　　处：《医用生物力学》2003年第3期129-135,共7页Journal of Medical Biomechanics

基　　金：国家自然科学基金会项目 (39970 194 )

摘　　要：目的　探讨川芎嗪和剪切率对悬浮血管内皮细胞凋亡的影响。方法　体外培养大鼠脑微血管内皮细胞 (rCMEC) ,以川芎嗪和锥 板旋转剪切方式共同作用于悬浮rCMEC ,采用AnnexinV FITC/PI双染法 ,流式细胞仪检测川芎嗪和剪应力对rCMEC凋亡的影响。结果　方差分析表明 ,川芎嗪和剪切率对rCMEC凋亡率有显著影响 ,川芎嗪降低凋亡率 ;剪切率增加凋亡率 ,二者作用相反但无交互作用。组间t检验表明 :单独使用川芎嗪时 ,在本实验剂量范围内 (3 1 .5～ 1 2 6μg/ml) ,川芎嗪对静态悬浮rCMEC凋亡率无显著影响。高剪切率 (60 0 0 ,80 0 0s- 1)可诱导rCMEC凋亡 ,川芎嗪对此具有显著抑制作用。结论　适当剂量的川芎嗪可用于抑制高剪切率诱导的血管内皮细胞凋亡。Objective To study the effects of ligustrazine and shear stress on the apoptosis of suspending vascular endothelial cell. Methods Rat cerebral microvascular endothelial cell (rCMEC) cultured in vitro was used. The administration of the drug incorporated with different levels of shear rate introduced by rotational cone plate shear generator. The influences of ligustrazine and shear rate on the apoptosis were determined by the immunofluorescent of Annexin V-FITC/PI with flow cytometer. Results The analysis of variance shows that ligustrazine and shear rate can affect the apoptosis rate of suspending rCMEC significantly. Ligustrazine can restrain apoptosis rate, high shear rates can raise apoptosis rate;however, there is no interaction between ligustrazine and shear rate. The t test shows that ligustrazine(31.5～126μg/ml) has no significant effect on suspending rCMEC apoptosis at zero shear rate. However, high shear rates (6000, 8000s -1 ) can induce apoptosis which can be significantly inhibited by adding ligustrazine. Conclusion The results indicate that a suitable dose of ligustrazine can distinctly restrain vascular endothelial cell apoptosis induced by high shear rates.

关 键 词：川芎嗪 剪切率 血管内皮细胞 凋亡 流式细胞仪 

分 类 号：R318.01[医药卫生—生物医学工程]