情绪应激对不同脑区c-fos表达的影响  

THE EFFCET OF EMOTIONAL STRESS ON THE C-FOS GENE EXPRESSION OF BRAIN AREAS

作　　者：邵枫[1] 林文娟[2] 王玮雯[2] 陈极寰[2] 

机构地区：[1]北京大学心理学系,北京100871 [2]中国科学院心理研究所脑-行为中心,北京100101

出　　处：《心理学报》2003年第5期685-689,共5页Acta Psychologica Sinica

基　　金：中科院知识创新工程项目 (Kscx2 2 0 3 );国家自然科学基金资助项目 (3 0 2 0 0 0 80 );教育部科学技术重点项目 (0 2 170 )

摘　　要：利用电击信号和空瓶刺激两种情绪应激体液免疫调节作用动物模型 ,以c -fos原癌基因为探针 ,观察情绪应激后 2个小时 ,大鼠全脑的c -fos原癌基因表达情况 ,探讨情绪应激对不同脑区c -fos表达的影响。结果表明 ,电击信号和空瓶刺激两种情绪应激源均能引起某些脑区或核团的c -fos蛋白表达明显增加 ,包括额皮质、扣带皮质、杏仁内侧核、前连合核、下丘脑背内侧核弥散部、弓状核、孤束核。结果提示 。To explore the effect of emotional stress on c-fos gene expression of brain area s, two types of animal models of immunomodulation induced by emotional stressor were used. One was footshock apparatus as signal stimuli to cause the rats which were given footshock before emotional stress, the other was empty water bottle to cause the rats which were trained to drink water at two set times each day em otional stress. Both of the emotional stressors were demonstrated to suppress th e primary immunity of rats. The c-fos gene, a nuclear proto-oncogene, is transie ntly expressed in neurons after stimulation and within one hour produces detecta ble amounts of its product, c-fos. Immunocytochemical localization of the c-fos protein was used to determine neuronal activation in areas of the central nervou s system. After two hours of emotional stress, the rats were perfused transcardi ally, then the brains were removed, blocked, immersed, cut with a freezing micro tome, immunocytochemical stained.The results showed that compared to rats in con trol groups, c-fos protein was strongly expressed at several brain areas in rats of the emotional stressed group. The brain areas contain frontal cortex, cingul um cortex, amygdala, anterior commissure nucleus, arcuate nucleus, diffuse part of dorsalmedial nucleus hypothalamus, solitary nucleus. Conclusion: These brain areas maybe the major central areas activated by the emotional stress.

关 键 词：情绪应激 C-FOS原癌基因 脑区 

分 类 号：B845[哲学宗教—心理学]