异种胸腺修饰的可行性研究  

Feasibility of intrathymic inoculation with xenogeneic antigen

作　　者：倪斌[1] 沈振亚[1] 张治[1] 瞿冀琛[1] 何建明[1] 孟自立[1] 

机构地区：[1]苏州大学附属第一医院心胸外科器官移植实验室,215007

出　　处：《中华实验外科杂志》2003年第8期742-744,共3页Chinese Journal of Experimental Surgery

摘　　要：目的探讨非协调性异种小动物模型中异基因胸腺修饰的可行性.方法供体三色豚鼠和受体SD大鼠各10只随机平均分成对照组(A组)和实验组(B组).A组大鼠接受3.5Gy全身照射,B组大鼠在此基础上3 d后接受5×106个豚鼠脾淋巴细胞胸腺内注射;两组大鼠分别在照射后18d接受豚鼠脾淋巴细胞腹腔内注射.结果接受胸腺注射后15d,B组混合淋巴细胞培养刺激效应低于A组(P＜0.05);3周后B组大鼠胸腺细胞表面仍有较高的豚鼠MHC-Ⅱ类抗原表达;腹腔注射后,B组中IgM类天然抗体水平的上升与A组相似,而IgG类上升明显受抑(P＜0.01).结论豚鼠-大鼠异种胸腺修饰可使受体新生T细胞对注入的抗原产生免疫抑制.Objective To investigate the effect of T cells on the delayed xenograft rejection by in- trathymic injection of xenogeneic antigen in the discordant xenotransplantation from Guinea-pig to rat. Methods Guinea-pig and rat were, respectively, selected as donor and recipient. Denor (guinea-pig) and recipient (SD rat) were divided randomly into two groups; the control group (group A) and the experi- ment group (group B). All rats of two groups received whole body irradiation (3. 5 Gy) and were in- traperitoneally injected with guinea-pig spleen cells (5×10~6) 18 days after irradiation for observing the change of the natural antibody (IgM and IgG) in group B. Three days after irradiation, rats received in- trathymic injection. Results The results of MLR showed that the stimulative effect in group A was stronger than in group B (P < 0. 05) after 15 days of intrathymic injection. The expression of the MHC- Ⅱ antigen of guinea-pig on rat's thymus cellular surface was (17. 02±5. 13)% after 3 weeks in group B. After one week of intraperitoneal injection, in group A the level of natural antibody IgM and IgG was ascended Obviously, but in group B, IgM elevated also, but IgG didn' t significantly raise (P < 0. 01). Conclusion In the model of discordant xenotransplantaion from guinea-pig to rat, intrathymic inocula- tion could induce immune suppression of T cells.

关 键 词：异种胸腺 修饰 可行性研究 三色豚鼠 胸腺移植 脾淋巴细胞 胸腺内注射 

分 类 号：R392[医药卫生—免疫学]