山东泰安地区TTV检测和部分基因克隆及序列测定  

Detection of transfusion transmitted virus(TTV)and partial gene cloning and sequencing in the Taian areas of Shandong

作　　者：于建国[1] 商庆华[1] 孙思才[1] 任诰[2] 肖德明[3] 尹燕明[4] 

机构地区：[1]中国人民解放军第88医院,山东泰安271000 [2]上海第二军医大学 [3]泰安市中心医院 [4]泰山医学院附属医院

出　　处：《山东医药》2003年第22期19-21,共3页Shandong Medical Journal

摘　　要：目的　探讨山东非甲 -庚肝炎和肝癌患者输血传播病毒 (TTV)感染状况和基因变异情况。方法　应用逆转录聚合酶链反应 (PCR)检测 2 6例山东泰安地区非甲 -庚型肝炎和 12例肝细胞癌 (HCC)患者血清中TTV DNA ,并对阳性扩增的产物利用 PCR技术片段直接克隆和测序 ,分析其基因变异的情况。结果　 2 6例非甲 -庚型肝炎中 11例 TTVDNA阳性 (42 .3%)。对其中两株 (TTVSD4、TTVSD5 )部分基因克隆测序 ,并与日本株(ABOO8394)相比较 ,其核苷酸序列同源性分别为 99.9%和 10 0 %。而 12例肝细胞癌患者中 3例 TTVDNA阳性(2 5 .0 %) ,对其中一株 (TTVSD6 )部分基因克隆与测序 ,与日本株 (ABOO8394)相比较 ,其核苷酸序列同源性为99%;TTV山东泰安三株间核苷酸同源性均为 99%。结论　研究证实山东泰安地区非甲～庚型肝炎和肝细胞癌患者中存在着较高 TTV感染 ,TTV感染可能具有嗜肝性 ,而且可能与肝功能损害有关 ,是引起非甲～庚型肝炎的重要病原。Objective To understand the prevalence of TTV infection and genetic variation of TTV isolated in Shandong Province.Methods TTVDNA were amplified and detected by PCR from sera of patients with NonA-G hepatitis and hepatic cancer. The PCR products were cloned and sequenced.Results TTVDNA was positive in 11 of 26 patients(42.3%) with NonA-G hepatitis. Partial gene of two TTV isolated(TTVSD4,TTVSD5) was cloned, sequenced and compared with known sequence of TTV isolates in Japan(ABOO8394) reported by Okamoto. The nucleotide homology were 99% and 100%. TTVDNA was positive in 3 of 12 patients (25%) with hepatic cancer. Partial gene of a TTV isolated (TTVSD6) was cloned, sequenced and compared with known sequence of TTV isolated in Japan(ABOO8394) reported by Okamoto. The nucleotide homology was 99%..Conclusions The ratio of TTV infection is high in the Taian areas of Shandong. TTV might be hepatotropic, and it probably correlated with the impairment of liver function. So it is likely to be the pathogen of NonA-G hepatitis.

关 键 词：非甲-庚肝炎 肝癌 输血传播病毒感染 TTV检测 基因克隆 序列测定 

分 类 号：R512.6[医药卫生—内科学] R735.7[医药卫生—临床医学]