高效液相色谱法测定洁肤霜中氯霉素的含量  

Determination of Chloromycetin in Jiefushuang with HPLC

作　　者：王亚南[1] 嵇扬[1] 贺吉川[2] 

机构地区：[1]中国人民解放军总后勤部卫生部药品仪器检验所,北京100071 [2]中国人民解放军北京军区联勤部药检所,北京100071

出　　处：《中国卫生检验杂志》2003年第4期434-435,共2页Chinese Journal of Health Laboratory Technology

摘　　要：〔目的〕建立高效液相色谱法测定洁肤霜中氯霉素的含量。〔方法〕采用四氢呋喃作溶剂直接将洁肤霜溶解 ,用乙腈— 0 0 5mol L磷酸盐缓冲液 (4 0 :60 )为流动相 ,检测波长 2 40nm。〔结果〕线性范围内相关系数r为 0 9998,回收率分别为 10 0 6% ,RSD分别为 1 3 %。〔结论〕HPLC法可用于洁肤霜中氯霉素的含量测定 ,方法准确 ,灵敏 ,专属性强 ,适用于产品的质量控制。ObjectiveAIM The HPLC method was established for determination of chloromycetin and in JIEFUSHUANG 〔Methods〕 Tetrahydrofuran was selected as solvent, the mixture of methylcyanide- phosphate buffer solution(40:60) was used as mobile phase The detection wave length was 240nm 〔Results〕 The method was linear with the linear coefficient of 0 9998 The average recovery was 100 6%, the relative standard deviation was 1 3% 〔Conclusions〕 The method is accurate,sensitive for the analysis of chloromycetin in JIEFUSHUANG

关 键 词：高效液相色谱法 含量测定 洁肤霜 氯霉素 四氢呋喃 

分 类 号：R194.7[医药卫生—卫生事业管理] TS974.1[医药卫生—公共卫生与预防医学]