利用cDNA微阵列技术筛选成年期与胚胎期睾丸差异表达基因——钙联接蛋白基因  

Molecular Cloning and Identification of Calmegin Gene in Embryo and Adult Testes by cDNA Microarray Hybridization

作　　者：崔婷[1] 祝辉[2] 王黎熔[2] 

机构地区：[1]南京医科大学第一附属医院检验科,江苏南京210029 [2]南京医科大学江苏省生殖医学重点实验室,江苏南京210029

出　　处：《中华男科学杂志》2003年第5期355-357,360,共4页National Journal of Andrology

摘　　要：目的 :用cDNA微阵列技术克隆和筛选成年期与胚胎期睾丸差异表达基因 ,以进行睾丸发育及精子发生的调控研究。　方法 :构建人睾丸cDNA芯片 ,分别用正常成人及胚胎睾丸的mRNA探针进行杂交 ,对成人和胚胎睾丸中差异表达的基因进行高流量的比较。　结果 :发现 1条成人睾丸高表达、胚胎低表达的基因———钙联接蛋白 (CLGN)基因。　结论Objective: To identify gene differential expression in embryo and adult testes by cDNA microarray techniques. Methods: cDNA probes of embryo and adult testes were used to hybridize the cDNA microarray of human testis, and the clones of differential hybridization were sequenced and analyzed. Results: The calmegin ( CLGN ) gene which had been confirmed to be involved in spermatogenesis was found, and it exhibited more than 20 fold difference at expression level between adult and embryo human testes. Conclusion: Genes differential expression in adult and embryo human testes can be identified by cDNA microarray hybridization.

关 键 词：睾丸 CDNA微阵列 钙联接蛋白基因 

分 类 号：R346[医药卫生—基础医学]