男性性病病人穿通支原体nPCR检测  

nPCR DETECTION OF MGCOPLASMA PENETRANS AMONG MALE PATIENTS WITH SEX TRANSMITTED DISEASES

作　　者：孙蓉[1] 孙峰[1] 朱进 张新华 时祝帅 

机构地区：[1]扬州大学医学院,225001 [2]扬州市皮肤病防治所

出　　处：《现代预防医学》2003年第4期492-493,共2页Modern Preventive Medicine

摘　　要：目的 :探讨扬州地区男性性病病人穿通支原体 (Mpe)感染状况及 Mpe与 STD感染的关系。方法 :应用套式PCR (n PCR)对扬州地区的 76例男性 STD者的尿道拭子进行 Uu、Mh、Mpn、Mg、Mf、Mpe、Mpi等七种支原体联合检测 ,并对 Mpe阳性标本作了 DNA测序分析。结果 :76例男性 STD患者中有 2例 Mpe阳性 ,阳性率为 2 .6 %。其中 1例经DNA测序与 Mpe(GTU5 4 T株 )标准株完全一致。结论 :Mpe是 HIV感染的协同因子和 AIDS发病的诱发因素 ,在 STD患者尿道拭子标本检出 Mpe值得关注。Objective:To research the infection of Mycoplasma penetrans(Mpe) of the male sex transmit diseases(STDs) in Yangzhou and relation between Mpe and STDs.Methods:Using Nested Polymerase Chain Reaction(PCR) to detect the Uu?Mh?Mpn?Mg?Mf?Mpe?Mpi seven grow mycoplasmas of 76 mens urethra specimen from region of Yangzhou.DNA sequencing was detected.Results:Two of male 76 sex transmitted diseases were positive the positive rates of Mpe was 2.6%.One of them,the DNA sequence was the same as Mpe(GTU54 T) of modle stem.Conclusion:The Mpe is cooperating gene of HIV-infected and occasion of AIDS.That Mpe detected in the male STDs urethra specimen is worth noticing.

关 键 词：男性 性病 检测 穿通支原体 套式聚合酶链反应 

分 类 号：R759[医药卫生—皮肤病学与性病学] R446.5[医药卫生—临床医学]