尿标本细菌高渗培养及其药物敏感试验结果分析  

Analysis of Bacteria Hyperosmotic Cultivation and its Drug Sensitive Test in Urine Samples

作　　者：顾孔珍[1] 吴水河[2] 

机构地区：[1]中南大学医学技术与情报学院临床微生物学与免疫学教研室,中国湖南长沙410013 [2]中南大学湘雅医院卫生部肝胆肠外科研究中心

出　　处：《实用预防医学》2003年第4期512-514,共3页Practical Preventive Medicine

摘　　要：目的　探讨泌尿道感染者尿标本中细菌的种类及其对抗生素的敏感性。　方法　将 86 4份尿标本进行高渗培养 ,同步进行普通培养 ,用 API细菌鉴定系统进行种属鉴别 ;然后用 K- B纸片扩散法进行药敏实验。　结果　高渗培养分离出病原菌 192株 (占 2 2 .2 % ) ,普通培养分离出病原菌 93株 (占 10 .8% )。肠球菌、大肠埃希菌、表皮葡萄球菌、克雷伯肠炎杆菌 4种主要病原菌共同敏感的药物是呋喃妥因 ,舒普深 ;对复方新诺明均耐药。　结论　泌尿道病原体检测进行高渗培养十分必要 ,可提高细菌检出率 。Objective To explore the bacteria in urine of the patients with urinary infection and its drug sensitivity. Methods Hyperosmotic and routine cultivation and drug sensitivity test were performed on the urine samples of 864 patients with urinary infection. Result 192 strains of bacteria were seperated out from these specimens on hyperosmotic cultivation and 93 strains of bacteria were separated out on routine cultivation. On drug sensitive test:E.raffinosus,E.coli,S.epidermidis,K.peneumoniae were all sensitive to Furadantin and Cefoperazone and were resistant to SMZ. Conclusion Hyperosmotic cultivation is very important in urinary infection. It increases the positive rate of bacteria separated and provides an important clue to the diagnosis and rational administration of antibiotics.

关 键 词：尿标本 高渗培养 药敏分析 

分 类 号：R969.4[医药卫生—药理学]