Lin^-CD_(34)^-与Lin^-CD_(34)^+细胞差异表达基因的鉴定  

Identification of differentially expressed genes in Lin^-CD_(34)^-and Lin^-CD_(34)^+ cells

作　　者：王冬梅[1] 杨莉[1] 李梁[1] 冯凯[1] 白慈贤[1] 陈琳[1] 裴雪涛[1] 

机构地区：[1]军事医学科学院野战输血研究所,北京100850

出　　处：《中华血液学杂志》2003年第8期423-425,共3页Chinese Journal of Hematology

基　　金：国家重点基础研究发展规划( 973)资助项目(G19990 5390 3);国家杰出青年基金资助项目( 3982 5111);国家自然科学基金资助项目 ( 30 2 0 0 113)

摘　　要：目的　克隆并鉴定Lin-CD3 4-细胞区别于Lin-CD3 4+细胞的可能与其性状相关的基因。方法　以Lin-CD3 4-细胞作为检测对象 ,Lin-CD3 4+细胞作为驱动细胞 ,应用抑制消减杂交技术构建Lin-CD3 4-细胞的cDNA消减文库。选取部分克隆测序 ,测序结果与GenBank进行同源性比较和功能分析。结果　共获得 593个含有插入片段的克隆 ,片段的长度为 30 0～ 50 0bp。随机选取 53条EST进行测序 ,其中 37条EST代表了 1 0个已知基因 ,其余 1 6条EST代表了 4个未知序列。结论　利用抑制消减杂交技术鉴定出部分Lin-CD3 4-细胞特异表达的基因 ,可能与Lin-CD3Objective To identify genes that differentially express ed in Lin -CD 34 - and Lin -CD 34 + cells.Methods W ith Lin -CD 34 - cells as tester and Lin -CD 34 + cells as driver, cDNA subtractive library for Lin -CD 34 - cells was constructed using su ppression subtractive hybridization technique. Part of clones in the library wer e sequenced and the homologue analysis was conducted against the DNA database in GenBank.Results 593 clones containing an average of 300～500? bp insert were identified.Of them, 53 randomly selected ESTs were sequenced. Hom ologue analysis revealed that 37 ESTs represented 10 known genes, and the other 16 ESTs represented 4 novel sequences.Conclusion Part of specif ically expressed genes in Lin -CD 34 - cells were identified, which maybe related to Lin -CD 34 - cells’ specific characteristics.

关 键 词：Lin^-CD34^-细胞 Lin^-CD34^+细胞 造血干细胞 抑制消减杂交技术 差异表达基因 

分 类 号：R346[医药卫生—基础医学]