北京株和广州株SARS病毒与非典型肺炎患者血清中和抗体反应观察  

Cross neutralization reaction of SARS virus BJ01 and GZ01 strains with sera of SARS patients from Beijing and Guangzhou regions

作　　者：彭文明[1] 常国辉[1] 刘洪[1] 于曼[1] 谭刚[1] 范宝昌[1] 姜涛[1] 邓永强[1] 段鸿元[1] 祝庆余[1] 秦鄂德[1] 

机构地区：[1]军事医学科学院微生物流行病研究所,北京100071

出　　处：《中华微生物学和免疫学杂志》2003年第7期495-497,共3页Chinese Journal of Microbiology and Immunology

基　　金：国家 8 63高技术资金资助项目 ( 2 0 0 3AA2 0 82 0 1)

摘　　要：目的　研究北京和广州地区分离的SARS病毒的抗原性关系 ,为非典型肺炎疫苗候选株的筛选提供依据。方法　采用Reed Muench法测定BJ0 1和GZ0 1株病毒的滴度 ,分别选取北京和广州地区SARS病人血清各 6份 ,采用固定病毒 稀释血清法进行微量细胞交叉中和试验 ,测定两地病人血清对BJ0 1和GZ0 1株病毒的中和抗体水平及交叉中和能力 ,分析这两株SARS病毒抗原性的差异。结果　北京和广州两地的非典型肺炎病人血清能够中和本地分离的SARS病毒。两地血清可互相交叉中和BJ0 1和GZ0 1株SARS病毒。中和抗体水平相同。结论　北京和广州两地分离的SARS病毒BJ0 1和GZ0 1株抗原性相似、差异较小。Objective To study the immunogenic relationship between SARS virus BJ01 and GZ01 strains, for selecting vaccine candidate virus strain. Methods Using Reed-Muench method to test the titers of SARS virus BJ01 and GZ01 strains. 6 sera from SARS patients of Beijing and Guangzhou were separately selected and cross-neutralization test was conferred to investigate their neutralization antibody titer and ability of cross neutralization. Results ① The sera of Beijing and Guangzhou patients could neutralize local isolated SARS virus. ② The sera of Beijing and Guangzhou patients could cross-neutralize both BJ01 and GZ01 strains. ③ Their neutralization antibody titers were almost the same with both virus strain. Conclusion The SARS virus BJ01 and GZ01 strains showed no distinct difference in terms of neutralization antibody reactivity.

关 键 词：严重急性呼吸综合征 SARS病毒 交叉中和试验 抗原性 

分 类 号：R511.9[医药卫生—内科学]