人巨细胞病毒感染对脐静脉内皮细胞粘附分子和核转录因子表达的影响  

The effect of human cytomegalovirus infection on the expression of adhesion molecular and nuclear transcription factor in cultured human umbilical vein endothelial cells

作　　者：程敏[1] 闻良珍[1] 凌霞珍[1] 赵捷[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科,武汉430030

出　　处：《中华微生物学和免疫学杂志》2003年第7期502-506,共5页Chinese Journal of Microbiology and Immunology

摘　　要：目的　研究人巨细胞病毒 (humancytomegalovirus,HCMV)体外感染对人脐静脉内皮细胞 (HUVEC)表面细胞间粘附分子 1(ICAM 1)mRNA和核转录因子kappaB(NF κB)表达的影响 ,探讨NF κB活化与ICAM 1mRNA表达的关系。方法　用RT PCR方法检测ICAM 1mRNA的表达 ,用凝胶电泳迁移率改变试验 (EMSA)检测NF κB活性。结果　HUVEC感染HCMV后 1h ,NF κB活性增加 (P <0 .0 5 ) ,感染后 2h ,ICAM 1mRNA表达增加 (P <0 .0 5 ) ,二者均于 8h达高峰 ,至 2 4h时仍维持较高表达(P <0 .0 1) ;加入NF κB活性抑制剂四氢吡咯二硫代氨基甲酸酯 (PDTC)后 ,NF κB活性明显减弱 ,同时ICAM 1mRNA的表达也明显降低 (P <0 .0 1)。结论　HCMV感染可激活NF κB ,使ICAM 1mRNA的表达增加 ,从而引导炎症反应至感染部位 ,并有利于病毒感染在细胞间扩散。NF κB参与ICAM 1转录的调控 ,其活性变化影响ICAM 1mRNA的表达。Objective To investigate the effect of human cytomegalovirus (HCMV) infection on the expression of intercellular adhesion molecule-1 (ICAM-1) mRNA and nuclear factor kappa B (NF-κB) in human umbilical vein endothelial cells (HUVEC) so as to explore the relationship between the activation of NF-κB and the expression of ICAM-1 mRNA. Methods HUVEC was cultured and then infected by HCMV. Expression of ICAM-1 mRNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Activity of NF-κB was measured by electrophoretic mobility shift assay (EMSA). Results One hour after infection (h.p.i) by HCMV, the activity of NF-κB increased significantly as compared with mock-infected cells (P<0.05); at 2nd h.p.i, the expression of ICAM-1 mRNA also enhanced (P<0.05); both reached the peak at 8 h.p.i and remained relatively high level at 24th h.p.i (P<0.01). When the specific NF-κB inhibitor pyrrolidine dithiocarbamate (PDTC) was added, the activity of NF-κB dramatically decreased while the expression of ICAM-1 mRNA also decreased significantly (P<0.01). Conclusions The infection with HCMV may activate NF-κB and thereby enhance the expression of ICAM-1 mRNA in HUVEC which target the inflammatory reaction to sites of infection and facilitate the dissemination of HCMV among HUVEC. NF-κB may play a role in the regulation of the transcription of ICAM-1 and the change of its activity may influence the expression of ICAM-1 mRNA.

关 键 词：人巨细胞病毒感染 脐静脉内皮细胞 细胞间粘附分子-1 核转录因子-KB 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]