特发性血小板减少性紫癜患者外周血淋巴细胞协同刺激分子的表达  

Expression of costimulatory molecules on peripheral blood lymphocytes of patients with idiopathic thrombocytopenic purpura

作　　者：赵艳霞[1] 高清平[1] 陈友华[1] 敖绪斌[1] 陈伦华[1] 杨年兰[1] 王琼玉[1] 沈立云[1] 

机构地区：[1]武汉大学人民医院血液科,430060

出　　处：《中华血液学杂志》2003年第9期474-476,共3页Chinese Journal of Hematology

摘　　要：目的　探讨T、B淋巴细胞表面协同刺激分子在特发性血小板减少性紫癜 (ITP)的变化。方法　用流式细胞术检测 2 1例ITP患者和 9名正常人外周血淋巴细胞协同刺激分子B7 CD2 8和CD4 0 表达率 ,用ELISA方法检测ITP患者外周血PAIgG的含量。结果　ITP患者外周血CD4+ CD2 8+ 细胞率明显低于正常对照 ,CD86+ 、CD1 9+ CD86+ /CD1 9+ 细胞率明显高于对照组 (P <0 .0 5 ) ,而CD2 8+ 、CD4 0+ 、CD80+ 、CD1 9+ CD80+ 、CD1 9+ CD86+ 、CD1 9+ CD4 0+ 、CD4+ CD2 8+ /CD4+ 、CD1 9+ CD80+ /CD1 9+ 、CD1 9+ CD4 0+ /CD1 9+ 细胞率与对照组相比差异无显著性。 2 1例ITP患者中 16例PAIgG的含量高于正常 ,平均值为(184 .6 2± 38.0 0 )ng 10 7血小板 ,且PAIgG的含量与CD1 9+ CD86+ /CD1 9+ 细胞率呈正相关。结论　ITP患者CD4+ T淋巴细胞表面的协同刺激分子CD2 8表达无缺陷。PAIgG与B淋巴细胞表面的协同刺激分子CD86 的改变明显相关 ,因此CD86 的改变可能参与ITP自身免疫的病理机制 ,并为ITP患者单克隆抗体治疗提供理论指导。Objective To investigate the expressions of costimulators on peripheral T and B lymphocytes in patients with idiopathic thrombocytopenic purpura(ITP). Methods The expression of B7-CD_ 28 and CD_ 40 of peripheral lymphocytes was measured by flow cytometry in 21 ITP patients and 9 normal subjects. The expression of PAIgG was measured by ELISA method. Results The expression of CD_4 +CD_ 28 + was lower in ITP patients than in normal controls, but the expression of CD_ 86 + and CD_ 86 +CD_ 19 + was higher in ITP patients than in normal controls, while the expression of CD_ 80 +,CD_ 40 +,CD_ 28 +,CD_ 19 +CD_ 86 +,CD_ 19 +CD_ 40 +,CD_4 +CD_ 28 +/CD_4 +,CD_ 19 +CD_ 80 +/CD_ 19 + and CD_ 19 +CD_ 40 +/CD_ 19 + in ITP patients was normal. The PAIgG level was higher in 16 patients with a mean of (184.62±38.00) ng/10 7 plt. A positive correlation was found between PAIgG and CD_ 19 +CD_ 86 +/CD_ 19 + expression. Conclusion There is no deficiency in expression of CD_ 28 on CD_4 + T lymphocytes in ITP patients. The change of CD_ 86 expression on B lymphocytes is possibly involved in pathophysiology of ITP, which may provide a theoretical instruction for ITP patients immunological therapy.

关 键 词：特发性血小板减少性紫癜 外周血淋巴细胞 B7-CD28 CD40 酶联免疫吸附试验 

分 类 号：R554.6[医药卫生—血液循环系统疾病]