HPLC拆分法测定甲磺酸帕珠沙星中左旋异构体含量  

Determination of Levo-Pazufloxacin Mesilate by HPLC Splitting Methods

作　　者：张兰华[1] 张俊虎[2] 王杰松[2] 

机构地区：[1]中国人民解放军第261医院,北京100094 [2]中国人民解放军第306医院,北京100101

出　　处：《解放军药学学报》2003年第4期300-301,共2页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的　甲磺酸帕珠沙星有效结构为左旋体 ,为了对其进行质量控制 ,建立高效液相色谱法拆分法测定含量。方法　采用C18柱 (5 μm .2 5 0× 4 .6mm) ,流动相为 1.32gL 苯丙氨酸、1.0g硫酸酮 ,加水溶解并稀释到 10 0 0ml-甲醇 (80∶2 0 ) ,流速 1.5ml/min ,检测波长 335nm。结果　HPLC法测定甲磺酸帕珠沙星左旋体含量 ,在 0 .0 5 96～ 0 .5 346mg·ml-1范围内线性关系良好 ,相关系数为 0 .9998。结论　采用HPLC拆分检测方法测定甲磺酸帕珠沙星中左旋体异构体的含量 ,方法简便 ,结果准确。Aim Since the levo-pazufloxacin mesilate is effective, a HPLC procedure was developed to detect the content of levo-isomer.Methods Diamonsil C 18 (250×4.6mm,5μm) was utilized as analytical column.The mobile phase contained[1.32g L phenylalanine,1.0g cupric sulfate,diluted to 1 000ml with water]-methanol(80∶20).The flow rate was 1.5ml/min,detection wavelength was 335nm.Results The method proved to be linear over the range of 0.059 6～0.534 6mg·ml -1 ,the correlation coefficient was 0.999 8.Conclusion The determination of levo-pazufloxacin mesilate with HPLC splitting procedure was simple and accurate.

关 键 词：HPLC 拆分法 含量测定 甲磺酸帕珠沙星 左旋异构体 质量控制 高效液相色谱法 

分 类 号：R978.19[医药卫生—药品] R927.2[医药卫生—药学]