新化合物哌芳安他对血管内皮细胞氧化损伤的保护作用  

Protective Effect of Pivanampeta on Endothelial Cells from Oxidative Injury

作　　者：石翠格[1] 胡刚[1] 汪海[1] 

机构地区：[1]中国人民解放军军事医学科学院毒物药物研究所,北京100850

出　　处：《解放军药学学报》2003年第4期255-258,共4页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：国家重点基础研究发展规划项目 (No.GT19980 5 1112 )

摘　　要：目的　研究新化合物哌芳安他对培养的牛主动脉内皮细胞氧化损伤时ICAM - 1、MCP - 1mRNA表达水平的影响 ,探讨其抗动脉粥样硬化的作用机制。方法　使内皮细胞与不同浓度哌芳安他预孵育 2 0h ,再给予氧化低密度脂蛋白损伤 ,提取各组细胞总RNA ,通过逆转录 -聚合酶链式反应技术 ,以 β2 -微球蛋白为内参照检测内皮细胞ICAM - 1、MCP - 1mRNA表达水平的变化。结果　内皮细胞与哌芳安他预孵育后 ,再受到氧化损伤时 ,其ICAM - 1、MCP - 1mRNA的表达水平明显低于与ox -LDL直接接触的细胞。结论　哌芳安他在一定程度上可保护内皮细胞使之免于或减轻氧化损伤。Aim To investigate the effects of pivanampeta against ICAM-1 and MCP-1 mRNA expression in cultured endothelial cells derived from bovine aorta.Methods The ICAM-1 and MCP-1 mRNA expression in bovine aorta endothelial cells was measured by reverse transcription -polymerase chain reaction and normalized to the mRNA level of β 2 -microglobulin in the absence or in the presence of ox-LDL or land pivanampeta Results Both ICAM-1 and MCP-1 mRNA expression in the endothelial cells derived from bovine aorta were increased by ox-LDL stimulation,which could be prevented by pivanampeta at the concentrations of 10 and 100μmol·L -1 .Conclusions The over expression of ICAM-1 and MCP-1 in endothelial cells induced by ox-LDL could be blocked by pivanampeta.Pivanampeta can protect the endothelial cells from the oxidative injury.

关 键 词：化合物 哌芳安他 血管内皮细胞 氧化损伤 血管保护 动脉粥样硬化 

分 类 号：R543.5[医药卫生—心血管疾病]