Studies on Genetic Transformatiom of Embryogenic Suspension Cultures of Sweetpotato  

作　　者：ZHAI Hong and LIU Qing-chang(Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture/China Agricultural University , Beijing 100094 , P.R. China) 

出　　处：《Agricultural Sciences in China》2003年第7期791-797,共7页中国农业科学（英文版）

基　　金：supported by the National Natural Science Foundation of China(30170587);the National High Tech R&D Program,China(863 Program,2002AA241031,2001AA241181);the Teaching and Research Award Program for Outstanding Young Teachers in Higher Education Institutions of Ministry of Education,China.

摘　　要：Genetic transformation of embryogenic suspension cultures of sweetpotato cv. Lizixiang was conducted by using Agrobacterium tumefaciens strain A208SE harboring the binary vectors pROA93 with β-glucronidase(GUS)and neomycin phosphotransferase(NPTⅡ)genes. The results indicated that embryogenic suspension cultures precultured for 1 - 3 d were suitable for the transformation. The optimal cocultivation time was 4 - 5 d. The optimal concentration of kanamycin was 50-75 mg L-1 for suspension culture and 100 mg L-1 for embryogenic callus proliferation and plant regeneration. The optimal concentration of carbencillin was 100 mg L-1. Transgenic plants identified with GUS assays and PCR analyses were obtained.Genetic transformation of embryogenic suspension cultures of sweetpotato cv. Lizixiang was conducted by using Agrobacterium tumefaciens strain A208SE harboring the binary vectors pROA93 with β-glucronidase(GUS)and neomycin phosphotransferase(NPTⅡ)genes. The results indicated that embryogenic suspension cultures precultured for 1 - 3 d were suitable for the transformation. The optimal cocultivation time was 4 - 5 d. The optimal concentration of kanamycin was 50-75 mg L-1 for suspension culture and 100 mg L-1 for embryogenic callus proliferation and plant regeneration. The optimal concentration of carbencillin was 100 mg L-1. Transgenic plants identified with GUS assays and PCR analyses were obtained.

关 键 词：SWEETPOTATO Embryogenic suspension cultures A. tumefaciens-mediated method Transge-nic plants 

分 类 号：S531[农业科学—作物学]