8-Br-cAMP和槲皮素单独和联合应用对Eca-109细胞POLB及多药耐受相关蛋白的影响  

Effects of 8-Br-cAMP or quercetin alone or combined use on drug resistance in Eca-109 cells

作　　者：宫璀璀[1] 王文丽[1] 吴景兰[2] 刘影[1] 

机构地区：[1]解放军第153医院检验中心,郑州450042 [2]郑州大学分子细胞生物学研究中心,郑州450052

出　　处：《郑州大学学报（医学版）》2003年第5期701-702,共2页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省自然科学基金资助项目9840 2 180 0;河南省教育科研基金资助项目2 0 0 0 180 0 7

摘　　要：目的 :探讨 8 Br cAMP和槲皮素单独和联合应用对Eca 10 9细胞耐药性的影响。方法 :将培养的Eca 10 9细胞随机分为 4组 :① 8 Br cAMP组 (Br组 ) ;②槲皮素组 (Q组 ) ;③ (Br+Q)组 ;④不加药物的对照组 (C)组。同时培养 4 8h ,对以上 4组细胞制备细胞滴片及硝酸纤维素膜 (NCM)滴膜 2种标本 ,分别进行MRP和POLB的免疫组化染色和免疫斑点印迹阵列及TLC扫描。结果 :免疫组化染色显示 :C组的MRP IR、POLB IR强于Br组、Q组或 (Br+Q)组 ,P <0 .0 1。TLC扫描OD值 :C组MRP IR高于Br、Q(Br+Q)组约 3倍 ;POLB IR高于Br、Q或 (Br+Q)组约 2倍 ;MRP与POLB呈显著正相关r=0 .9838,P <0 .0 1。结论 :8 Br cAMP和槲皮素联合用药或单独用药均可降低Eca 10 9细胞的多药耐受性而提高药物的敏感性 。Aim:To study the effects of combined use of 8 Br cAMP (Br) and quercetin (Q) on drug resistance in Eca 109 cells. Methods: The cultured Eca 109 cells were randomly divided into 4 groups: Br, Q, (Br+Q) and C (no any drugs) groups and were cultured 48 h simultaneously. Both cell and NCM specimens for each group were prepared. The immunocytochemistry for MRP, POLB and immunoblotting with TLC scanning were performed respectively. Results: The immunoreactivity (IR) showed: a) MRP IR, C>Br, Q or (Br+Q) group, P <0.01; b) POLB IR, C>Br, Q or (Br+Q) group, P <0.01. There was a significant positive correlation between MRP and POLB. Conclusion: 8 Br cAMP, quercetin or combined use of 2 drugs could decrease the drug resistance in Eca 109 cells, which may be benefit to clinical therapy.

关 键 词：MRP POLB 8-BR-CAMP 槲皮素 ECA-109细胞 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]