扩张兔皮肤肌动蛋白、肌球蛋白和α-平滑肌肌动蛋白的变化  

Changes of actin, myosin, and α-SMA in expanded skin of rabbits

作　　者：王喜梅[1] 范志宏[2] 刘凯[3] 陈言汤[1] 

机构地区：[1]郑州大学第一附属医院整形外科,郑州450052 [2]上海第二医科大学附属仁济医院整形外科,上海200127 [3]上海第二医科大学附属第九人民医院整形外科,上海200011

出　　处：《郑州大学学报（医学版）》2003年第5期706-709,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：卫生部科研基金资助项目

摘　　要：目的 :了解兔皮肤扩张后皮肤肌动蛋白 (actin)、肌球蛋白 (myosin)和α 平滑肌肌动蛋白 (α SMA)的变化。方法 :选用 2～ 3kg新西兰大白兔 6 4只分为 2大组 ,快速扩张组和常规扩张组 ,每组 32只 ,每大组再分为 4组 ,为扩张完成后即时、1周、12周、2 4周组 ,每组 8只 ,其中 4只为实验组 ,另 4只植入扩张器不扩张作为对照组。通过免疫组织化学和原位杂交的方法观察扩张后即时、1周、12周、2 4周皮肤中actin、myosin、α SMA的变化。结果 :扩张后兔皮肤中的actin、α SMA阳性细胞数明显增多 ,快速扩张组与常规扩张组各时段比较差异有统计学意义。myosin快速扩张与常规扩张组差异无统计学意义。免疫组化和原位杂交的结果相同。结论 :扩张刺激可使成纤维细胞转化为肌成纤维细胞 ;快速扩张组αAim:To study the changes of actin, myosin, and α SMA expression after skin expansion in rabbits.Methods: A total of 64 New Zealand rabbits (2～3 kg) were allocated into rapid expansion and regular expansion groups. The changes of actin, α SMA, and myosin expression were observed with immunohistochemistry and in situ hybridization immediately and at 1, 12, 24 weeks after expansion.Results: The positive cells of actin and α SMA increased obviously in the skin after expansion (especially in rapid expansion group).The results from immunohistochemistry and in situ hybridization were almost the same.Conclusion: Fibroblast can turn into myofibroblast partially because of the expansion.α SMA positive cells in rapid expansion group is more than that in regular group, which may explain the theory of more rapid expansion, more serious contracture.

关 键 词：组织扩张术 挛缩 Α-SMA 兔 皮肤 肌动蛋白 肌球蛋白 Α-平滑肌肌动蛋白 

分 类 号：R622[医药卫生—整形外科]