扩张兔皮肤Ⅰ、Ⅲ型胶原的变化  

Changes of collagen Ⅰand Ⅲ in expanded rabbit skin

作　　者：王喜梅[1] 范志宏[2] 刘凯[3] 陈言汤[1] 

机构地区：[1]郑州大学第一附属医院整形外科,郑州450052 [2]上海第二医科大学附属仁济医院整形外科,上海200127 [3]上海第二医科大学附属第九人民医院整形外科,上海200011

出　　处：《郑州大学学报（医学版）》2003年第5期710-711,共2页Journal of Zhengzhou University(Medical Sciences)

基　　金：卫生部科研基金资助项目

摘　　要：目的 :探讨兔皮肤扩张后皮肤Ⅰ、Ⅲ型胶原的改变。方法 :选用 2～ 3kg新西兰大白兔 6 4只分为 2大组 ,快速扩张组和常规扩张组 ,每组 32只 ,每大组再分为 4组 ,为扩张完成后即时、1周、12周、2 4周组 ,每组 8只 ,其中 4只为实验组 ,另 4只植入扩张器不扩张作为对照组。通过免疫组化的方法观察扩张后即时 ,1,12 ,2 4周真皮中胶原含量、Ⅰ、Ⅲ型胶原比例的变化。结果 :扩张后即时Ⅰ型胶原染色的基底膜连续性破坏。排列无规律 ,多呈碎片状。胶原束间隙增大、Ⅲ胶原合成增加 ,快速扩张组比常规扩张组明显 ;后期胶原纤维重叠。结论 :胶原Ⅰ。Aim: To observe the changes of collagen Ⅰand Ⅲ in expanded rabbit skin.Methods: A total of 64 New Zealand rabbits (2～3 kg) were allocated into rapid expansion and regular expansion groups. The changes of skin collagens were observed immediately after expansion and at 1, 12, 24 weeks after expansion with immunohistochemistry. Results: The basilar membrane of collagen I was injured more seriously with an irregular arrangement. The synthesis of collagen Ⅲ increased immediately after expansion, and the space between collagens became larger, especially in rapid expansion group.Conclusion: The changes of collagenⅠand Ⅲ might be one of the direct reasons for contracture after skin expansion.

关 键 词：组织扩张术 挛缩 胶原 兔 皮肤 

分 类 号：R622[医药卫生—整形外科]