西沙比利对肝硬化大鼠小肠细菌及内毒素转位的影响  

Effects of cisapride on intestinal bacterial and endotoxin translocation in cirrhotic rats

作　　者：张顺财[1] 王唯[2] 任卫英[3] 周康[4] 贺伯明[5] 朱无难[1] 

机构地区：[1]复旦大学附属中山医院消化科,上海200032 [2]上海市中国科学院药物所 [3]复旦大学附属中山医院老年科,上海200032 [4]复旦大学附属中山医院实验研究中心,上海200032 [5]复旦大学附属中山医院内科实验室,上海200032

出　　处：《中华肝脏病杂志》2003年第9期539-541,共3页Chinese Journal of Hepatology

摘　　要：目的 研究西沙比利对肝硬化大鼠小肠细菌过度生长(IBO),细菌及内毒素转位,小肠转运时间及小肠通透性的作用。方法 正常对照组大鼠25只,肝硬化对照组大鼠25只,肝硬化治疗组20只(用等渗盐水),另20只肝硬化大鼠用西沙比利治疗,动物均测定各种参数如细菌和内毒素转位,小肠细菌过度生长,小肠转运时间及通透性。结果 48％肝硬化大鼠发生细菌转位,与无IBO肝硬化大鼠比较,IBO肝硬化大鼠的内毒素和细菌转位发生率高,肠转远时间延长,小肠通透性增高。BT的细菌与IBO主菌群一致。与对照组比较,西沙比利处理的肝硬化大鼠肠细菌和内毒素转位及IBO发生率降低,这与肠转运时间增快及通透性降低密切相关。结论 肝硬化大鼠内毒素和细菌转位可能是由于IBO和肠通透性增加的结果,而IBO的发生可能是由于肠转运时间延长所致。西沙比利可加速小肠转运时间,改善小肠通透性,这有助于防治小肠细菌和内毒素转位。Objective To further investigate the effects of cisapride on intestinal bacterial overgrowth (IBO), bacterial and endotoxin translocation, intestinal transit and permeability in cirrhotic rats. Methods 25 normal control rats, 25 cirrhotic rats, 20 cirrhotic rats received saline, and 20 cirrhotic rats treated with cisapride were included in the study. All animals were assessed with many variables including bacterial and endotoxin translocation, IBO, intestinal transit and permeability. Results Bacterial translocation was found in 48%(12/25) cirrhotic rats and none of control rats. Among the 20 rats with IBO, there were 11 rats with bacterial translocation (BT) while only one rats occurred BT out of the 5 rats without IBO. Cirrhotic rats with IBO had a significantly higher rate of endotoxin translocation, higher intestinal permeability and longer intestinal transit than those without IBO. BT of a specific organism was always associated with IBO of that organism. Compared with the placebo group, cisapride-treated rats had lower rates of bacterial and endotoxin translocation and IBO, which had close relationship with shorter intestinal transit and lower permeability. Conclusion Endotoxin and bacterial translocation in cirrhotic rats may be the result of IBO and higher permeability. IBO may be the result of longer transit. Cisapride which can accelerate intestinal transit and improve intestinal permeability is helpful in preventing and treating intestinal bacterial and endotoxin translocation.

关 键 词：西沙比利 肝硬化 大鼠 小肠细菌 内毒素转位 

分 类 号：R965[医药卫生—药理学]