石蜡包埋表皮组织中β-连环蛋白基因扩增技术的建立  

Amplification of β-catenin gene in the paraffin-embedded epidermal tissue

作　　者：陈翔[1] 谢红付[1] 陈服文[1] 

机构地区：[1]中南大学湘雅医院皮肤科,长沙410008

出　　处：《湖南医科大学学报》2003年第4期327-329,共3页Bulletin of Hunan Medical University

基　　金：国家自然科学基金资助项目 (30 2 0 0 2 48) ;湖南省自然科学基金资助项目 (0 2 jjy40 18) ;教育部留学回国人员科研启动基金 (2 0 0 3)

摘　　要：目的∶建立从石蜡包埋表皮组织中提取DNA ,进行 β 连环蛋白基因 3号外显子聚合酶链反应 (PCR)扩增的方法。方法∶从 14例不同重量、不同年代石蜡包埋皮肤鲍温氏病病变组织中提取DNA ,再进行 β 连环蛋白基因 3号外显子PCR扩增。结果∶5mg组织提取DNA的PCR扩增未获成功 ,而用 10mg和 2 0mg组织提取的DNA可成功扩增 ;已包埋 10年以上的 5例标本仅 2例成功扩增 ,而 9例 10年以内的标本全部成功。结论 :10mg及 10mg以上且 10年以内的石蜡包埋表皮组织可用于目标基因PCR扩增。Objective To establish a method of extracting DNA from the paraffin-embedded epidermal tissue and examine the amplification of β-catenin gene by polymerase chain reaction (PCR) analysis. Methods Exon 3 of the β-catenin gene was amplified by PCR in 14 paraffin-embedded Bowen’s disease (BD) of various weight and years. Results The amplification of exon 3 of β-catenin gene was successful in all the 10 mg and 20 mg samples, but not in the 5 mg samples. The amplification of exon 3 of β-catenin gene was successful in 9 samples embedded within 10 years, and in only 2 of the 5 samples embedded more than 10 years. Conclusion Epidermal tissue whose weight is 10 mg or more within 10 years of embedding can be used in the amplification of purpose gene by PCR.

关 键 词：石蜡包埋表皮组织 Β-连环蛋白 基因扩增技术 聚合酶链反应 肿瘤 

分 类 号：R346[医药卫生—基础医学]