白头翁中活性成分对荷瘤小鼠肿瘤的抑制作用  

The anti-tumor effect of the extract from Radix Pulsatillae

作　　者：冯丹[1] 钟长斌[2] 

机构地区：[1]华中科技大学同济医学院附属同济医院,湖北武汉430030 [2]南京中医药大学,江苏南京210029

出　　处：《中国医院药学杂志》2003年第9期532-533,共2页Chinese Journal of Hospital Pharmacy

摘　　要：目的 :研究从白头翁中提取的 2 3 羟基白桦酸的抗癌活性。方法 :从白头翁根的饮片中提取总皂苷 ,并经水解为苷元 ,柱层析法分离、纯化。用不同剂量的单体化合物食用植物油混悬液 ,分别给小鼠HepA肝癌和EAC腹水癌两种移植性肿瘤动物模型连续腹腔注射给药 10d ,观察其抑瘤效应及生命延长率。结果 :中、低剂量组对小鼠HepA实体瘤有显著抑制生长作用 ,但高剂量组反而作用不明显 ,各剂量组生命延长效果亦不明显。结论 :白头翁中提取的 2 3 羟基白桦酸在实验动物体内具有肿瘤抑制作用 ,但有一定毒性 。OBJECTIVE To extract anemosapogenin from Radix Pulsatillae and study its anti tumor activity.METHODS Total saponins were isolated from Radix Pulsatillae , then hydrolyze into sapogenins , the sapogenins were purified by column chromatography. The furified substance was mixed with vegetable oil as suspension of different concentrations. Mice with transplantable tumors(HepA liver carcinoma, Ehrlich ascites cancer)were administrated continuously for ten days by i.p. with different dosages according to the mice weight. The anti tumor effect in vivo and the rate of life elongation of the mice was observed. RESULTS Low concentration group and middle concentration group had obvious effect on inhibiting the growth of HepA liver carcinoma in vivo, but the high concentration group had little effect . However, the life elongation effect was not obvious.CONCLUSIONS The extract from Radix Pulsatillael (3β,23 dihydroxyl  20(29) lupene 28 acid)shows potent anti-tumor effect in vivo, but it has some toxicity, structure modification should be needed.

关 键 词：白头翁 23-羟基白桦酸 动物疾病模型 抑瘤率 生命延长率 抗肿瘤活性 

分 类 号：R965[医药卫生—药理学] R285.5[医药卫生—药学]