妊娠肝内胆汁淤积症胎盘细胞凋亡与Fas、FasL的研究  

Study on Apoptosis and Expression of Fas, FasL in Placenta of Intrahepatic Cholestasis of Pregnancy

作　　者：王冬梅[1] 腊晓琳[1] 丁丽[1] 平小佳[2] 

机构地区：[1]新疆医科大学第一附属医院产科,乌鲁木齐830054 [2]新疆医科大学第一附属医院病理科,乌鲁木齐830054

出　　处：《中华围产医学杂志》2003年第4期199-201,共3页Chinese Journal of Perinatal Medicine

摘　　要：目的　探讨妊娠肝内胆汁淤积症 (intrahepatic cholestasis of pregnancy,ICP)胎盘组织Fas及 Fas L (Fasligand)表达在细胞凋亡中的作用。　方法　采用 d U TP缺口末端标记法 (TU NEL )和免疫组化技术对 31例 ICP患者胎盘组织中的细胞凋亡、Fas、Fas L的基因表达进行检测 ,并以正常妊娠胎盘组织 31例作为对照组。　结果　 ICP组胎盘组织中细胞滋养细胞、合体滋养细胞、蜕膜细胞及绒毛间质细胞的凋亡指数 (AI)分别为 4 9.0 9± 9.13(t=13.4 1)、4 6 .6 4± 9.77(t=12 .16 )、35 .0 9±9.4 9(t=8.4 3)、38.74± 9.70 (t=11.2 8) ,明显高于对照组 (P<0 .0 0 5 )。 ICP组细胞滋养细胞和合体滋养细胞 Fas表达明显增强 (P<0 .0 0 5 ) ,Fas L在细胞滋养细胞、合体滋养细胞、蜕膜细胞及绒毛间质细胞中的表达明显低于对照组 (P<0 .0 0 5 )。　结论　 ICP患者胎盘组织中 Fas表达增强 ,Fas L表达减弱 ,可导致母胎间免疫耐受的破坏 ,胎盘细胞凋亡增加 ,可能与 ICP患者胎盘功能减退有关。Objective To study the role of gene Fas and FasL expression in apoptosis of plcenta with ICP. Methods TUNEL (TdT-mediated dUTP nick end labeling) and immuno-hisochemistry methods were used for 31 placental samples with ICP and 31 normal placental samples to detect apoptosis index (AI) and the expression of Fas and FasL in placental tissues. Results AI of cytotrophoblast, syncytotrophoblast, decidual cells and mediate cell(49.09±9.13,t=13.41;46.64±9.77,t=12.16;35.09±9.49,t=8.43;38.74±9.70,t=11.28) in ICP group was significantly higher than that of control group (P<0.005). The expression of FasL of these positions in ICP group was lower than that in control group (P<0.005) The expression of Fas in cytotrophoblast and syncytotrophoblast of ICP group were higher than that in control group (P<0.005). Conclusions Abnormal expression of Fas and FasL in placental tissue may destroy the immunologic tolerance between the mother and the fetus and the increasing apoptosis of placenta may relate to the placental dysfunction.

关 键 词：妊娠并发症 肝内胆汁淤积症 胎盘 细胞凋亡 Fas FASL TUNEL 免疫组化技术 

分 类 号：R714.25[医药卫生—妇产科学]