荔枝品种亲缘关系的AFLP分析  

Studies on Genetic Relationship among Litchi Varieties by Using AFLP

作　　者：易干军[1] 霍合强[1] 陈大成[2] 黄自然[2] 蔡长河[1] 邱燕萍[1] 

机构地区：[1]广东省农业科学院果树研究所,广州510640 [2]华南农业大学,广州510640

出　　处：《园艺学报》2003年第4期399-403,共5页Acta Horticulturae Sinica

基　　金：国家自然科学基金资助项目 (3 970 0 10 1);广东省自然科学基金资助项目 (970 678)

摘　　要：应用AFLP技术 ,对 39个荔枝品种进行了遗传多样性分析及分类研究。筛选出适于荔枝AFLP分析的最佳引物组合 3对 ,分别为EcoRIAAC +MseICTG ,EcoRIAGC +MseICAT ,EcoRIACC +MseICAT。在分子水平上 ,荔枝品种的遗传多样性并非形态学性状所体现的那样丰富。AFLP分析将 39个荔枝品种分成了 6组 ,与传统的以果皮龟裂片隆起类型为分类标准的分类没有一致性。初步建立起荔枝主要栽培品种的分子标记标准图谱。应用AFLP技术对来自两个不同地方的两个荔枝品种 (桂味、妃子笑 )进行了鉴别。Identification and classification of 39 litchi varieties were studied based on AFLP analysis.Three pair of primers( Eco RI AAC+ Mse I CTG, Eco RI AGC+ Mse I CAT, Eco RI ACC+ Mse I CAT)were screened from 64 pare of primers.The analysis of genetic diversity among litchi varieties indicated on morphological level less abundant than molecular level.The cluster analysis results showed that the 39 cultivars could be divided into 6 groups.It is different from the traditional classification system based on peel morphology including peel smoothness,scale size,scale arrangement.The results showed peel morphology can not be used for litchi varieties classification as the only criterion.The molecular marker standard map of the main litchi varieties was established.Two varieties( Litchi chinensis 'Guiwei'and Litchi chinensis 'Feizixiao')come from different area were identified.

关 键 词：荔枝 品种 亲缘关系 AFLP分析 遗传多样性 引物组合 形态学性状 果皮龟裂片 分子标记标准图谱 

分 类 号：S667.1[农业科学—果树学]