三种氯霉素ELISA检测试剂盒评价  

Evaluation on three kinds of ELISA kits for chloramphenicol

作　　者：周德刚[1] 方晖[1] 左文霞[1] 郭志红[1] 闫小峰[1] 

机构地区：[1]北京市兽药监察所,北京100012

出　　处：《中国兽药杂志》2003年第9期28-31,共4页Chinese Journal of Veterinary Drug

摘　　要：　本试验选择三种氯霉素检测试剂盒(分别产于美国(A)、英国(B)和德国(G))及不同的前处理方法对牛奶及鸡肝组织中的氯霉素残留进行检测。从B/Bo50%抑制浓度、检测范围、标准溶液浓度梯度、板内变异、板间变异、样品添加试验、实际样品检测等方面对三种试剂盒及前处理方法进行对比试验,结果表明试剂盒A、B、G对缓冲液配制的药物标准液的50%抑制浓度均低于1ng/mL;三种试剂盒的检测范围分别为0.1～2.0ng/mL(A),0.14～21.0ng/mL(B),0.05～4.05ng/mL(G);板内变异系数均小于11.3%,板间变异系数均小于17.4%;样品回收率在70%～120%之间。Three kinds of ELISA kits for chloramphenicol were tested respectively. Kit A was from EuroDiagnostica Company in America, kit B was from RANDOX company in Britain and kit G was from rBiopharm company in Germany. The results showed that the corresponding concentration with 50% inhibition of kit A, B or G was less than 1 ng/mL respectively. The measuring range of kit A was 0.1～2.0 ng/mL, kit B was 0.14～21.0 ng/mL and kit G was 0.05～4.05 ng/mL. the interassay variations was less than 11.3% and the intraassay variations was less than 17.4%. The recovery was 70%～120%.

关 键 词：氯霉素 ELISA检测试剂盒 药物残留分析 酶联免疫法 牛奶 鸡肝组织 

分 类 号：S859.84[农业科学—临床兽医学]