人工感染猪胎儿猪细小病毒的强毒复壮及其在猪肾原代细胞上的增殖  

Virulent porcine parvovirus rejuvenated by artificial infection swine fetus

作　　者：董齐[1] 符芳[1] 刘凤华[1] 周连华[1] 韩孝成[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2003年第5期369-371,共3页Chinese Journal of Preventive Veterinary Medicine

摘　　要：猪细小病毒 (PPV)强毒 ,在 1～ 5日龄仔猪肾原代细胞上传代 ,传至 2 0代左右毒力下降。为保持PPV强毒的毒力 ,我们用一头妊娠母猪 (妊娠 4 8d)做剖腹术。用大剂量的PPV细胞培养物注入猪胎儿的羊膜腔内 ,人工感染猪胎儿 ,病毒通过猪胎儿体内的组织进行复制 ,使毒力增强。术后 12d迫杀母猪 ,取胎儿分离PPV强毒。Porcine Parvovirus virulence was decreased when it was passed 20 generations in 1～5 day porcine kidney primary cell.We challenge adapted cell culture viruses into fetus amniotic cavity by the ventrotomy pregnant sow (pregnant 48 days),after challenged 12days,pregnant sow was slaughtered and viruses were isolated from infectious fetus.We made PPV virulence stronger by such means.

关 键 词：猪细小病毒 强毒 复壮 

分 类 号：S852.659.2[农业科学—基础兽医学]