具超强汞富集能力基因工程菌生长情况的研究  

Studies on growth situations of Hg-superaccumulating recombinant E.coli in heavy metal wastewater

作　　者：邓旭[1] 李清彪[1] 孙道华[1] 黄益丽[1] 王琳[1] 

机构地区：[1]厦门大学化工系,厦门361005

出　　处：《工业微生物》2003年第3期11-15,共5页Industrial Microbiology

基　　金：教育部优秀青年教师资助计划资助

摘　　要：对具超强汞富集能力的基因工程菌在重金属汞离子存在的水体中的生长状况进行了研究。实验所采用的基因工程菌由于外源基因的表达而对Hg2 + 产生了一定的耐受力 ,而且这种耐受力经IPTG诱导后还能得到进一步的增强 ,耐受能力从Hg2 + 浓度 5mg/L提高到 15mg/L。IPTG加入的时机对菌体的生长和耐受力的有效诱导是重要的。一般在OD6 0 0 为 0 .5～ 0 .8时加入 ,且加入后还应保证 2h左右的诱导时间。实验结果还表明菌体在生长过程中对碳源的要求很低。The growth situations of recombinant E.coli JM109 which can superaccumulate Hg 2+ from aqueous solution containing Hg 2+ were studied. The Hg 2+ resistance ability of the recombinant bacteria was observed due to the expression of exogenous genes, and the ability could be further improved from 5mg/L of Hg 2+ concentration to 15mg/L by means of IPTG induction. The suitable time to add IPTG was important for bacteria growth. Basically IPTG was supposed to be added as OD 600 reached 0.5～0.8. Furthermore, at least 2 hours of induction period of time was needed to ensure the effective induction of Hg 2+ resistance. The experiment results also showed that the recombinant bacteria could grow at a very low concentration level of carbon source.

关 键 词：富集能力 基因工程菌 生长情况 重金属废水 细菌培养 

分 类 号：X703[环境科学与工程—环境工程]