超高灵敏度电化学发光分析仪研究  

Study on a Sensitive Electrochemiluminescence Assay Device

作　　者：闫贵虹[1] 邢达[1] 谭石慈[1] 

机构地区：[1]华南师范大学激光生命科学研究所,广东广州510631

出　　处：《光电子．激光》2003年第9期958-960,共3页Journal of Optoelectronics·Laser

基　　金：广东省自然科学基金团队资助项目(015012);广东省环保局科技攻关资助项目([2000]138)

摘　　要：根据电化学发光(ECL)检测微量物质方法的原理,研制了一种超高灵敏度ECL分析仪。该仪器采用多孔状的圆片Pt电极作为工作电极(WE),利用光纤收集传导光信号并采用单光子计数手段,能够灵敏检测微量物质的浓度。结果显示,该仪器检测六水合二氯三联吡啶钌[Ru(bpy)3Cl2·6H2O]的极限达到10-18mol/L,检测线性范围可达到5个数量级(10-13～10-8mol/L)。Based on the principle of electrochemiluminescence (ECL) method used in microanalysis, a high sensitive ECL analyzer was developed. This analyzer could actualize microanalysis sensitively. A multi-hole disk platinum electrode was used as working electrode (WE). A fiber band was used to collect and transfer the photon signal and a single photon counter was used to count the photon number in the analyzer. The results show that the detection limit of the analyzer is 10-18 mol/L Ru(bpy)3Cl2&middot 6H2O. The dynamic linear range extended over five orders, from 10-13-10-18 mol/L.

关 键 词：电化学发光 分析仪 微量物质检测 单光子计数 六水合二氯三联吡啶钌 

分 类 号：O646[理学—物理化学]