HPLC法测定黄芪中黄酮类成分和黄芪甲苷的含量  

Determination of Astragaloside Ⅳ and Flavoniod from Radix Astragali by HPLC

作　　者：胡芳弟[1] 封士兰[1] 赵健雄[1] 张勇[1] 陈立仁[2] 

机构地区：[1]兰州医学院药学院,甘肃兰州730000 [2]中国科学院兰州化学物理研究所,甘肃兰州730000

出　　处：《分析测试技术与仪器》2003年第3期173-177,共5页Analysis and Testing Technology and Instruments

基　　金：甘肃省省长基金项目 (GS0 1 2 -A43 - 0 93 )

摘　　要：比较黄芪对照药材和甘肃省近20个乡镇大面积种植的黄芪中黄芪甲苷和2个黄酮类化合物的含量,确定甘肃黄芪质量评价指标 采用KromasilODS-1色谱柱,甲醇-水为流动相,梯度洗脱,流速为1.0mL/min,检测波长254nm测定黄酮类成分的含量;乙腈-水为流动相,等度洗脱,流速为1.0mL/min,检测波长为200nm测定黄芪甲苷的含量.黄酮类成分毛蕊异黄酮的线性范围为2.350×10-3～4.700×10-2μg,平均回收率为98.34%,RSD为2.00%;芒柄花素线性范围为:2.325×10-3～4.650×10-2μg,平均回收率为96.53%,RSD为1.63%;黄芪甲苷线性范围为1.215～6.075μg,平均回收率为101.47%,RSD为2.21%.该方法简便,可靠,准确,具有一定的实用性,可用于黄芪药材质量控制.To establish the evaluation of quality standard of Radix Astragali according to their contents of Astragaloside Ⅳ and two flavoniods in Radix Astragsli from Gansu province and the standard of Radix Astragali. High- performance- liquid- chromatograph(HPLC) on a Kromasil ODS column (5 μm ,4.6 mm×250 mm) with water- methanol gradient elution, at column temperature of 25 ℃, flowrate of 1.0 mL/min and wavelength at 254 nm for determenation of two flavoniods; with acetiontrile- water isocratic elution, at column temperature of 25 ℃, flowrate of 1.0 mL/min and wavelength at 200 nm for determenation of Astragaloside Ⅳ. Linear ranges of Astragaloside Ⅳ, 7- hydroxy- 4′- methoxyisoflavone and 7,3′- dihydroxy-4′- methoxy- isoflavone were 1.215～6.075 μg, 2.325×10-3～4.650×10-2 μg and 2.350×10-3 ～ 4.700×10-2 μg respectively. The average recovery were 101.47 %, 96.53 % and 98.34 % respectively, and relative standard deviations were 2.21 %, 1.63 % and 2.00 % respectively. This method was practical and provided a new idea to study the quality standard of traditional Chinese drug.

关 键 词：HPLC法 测定 黄芪 黄酮类成分 黄芪甲苷 含量 中草药 化学成分 质量标准 

分 类 号：R284[医药卫生—中药学] R282.71[医药卫生—中医学]