大孔吸附树脂分离纯化广山楂总黄酮的最佳工艺条件及参数探讨  

Discussion of the optimum conditions and parameters of separation and purification of total flavones from Malus doumeri( Bois) Chev by macroreticular resin

作　　者：陈洪涛[1] 刘源焕[1] 覃学谦[2] 蔡丹昭[3] 

机构地区：[1]广西中医药大学制药厂,南宁530023 [2]广西中医药大学第一附属医院 [3]广西医科大学

出　　处：《山东医药》2015年第8期7-10,共4页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(81360688);广西自然科学基金资助项目(2012GXNSFAA239008);广西壮族自治区卫生厅中医药民族医药自筹经费课题项目(gzzc1203);广西壮族自治区教育厅广西高等学校科研项目(201204LX195)

摘　　要：目的探讨大孔吸附树脂分离纯化广山楂总黄酮的最佳工艺条件及参数。方法以静态和动态两个方面的饱和吸附量及洗脱率为指标,比较非极性树脂D101、弱极性树脂AB-8、中极性树脂HPD-450用于广山楂总黄酮分离纯化的优劣,并通过考察总黄酮回收率,优化所选最佳树脂的吸附工艺参数。结果 D101、AB-8、HPD-450静态饱和吸附量分别为94.802、118.494、98.317 mg/g干树脂,静态洗脱率分别为83.434%、91.253%、86.831%,洗脱液总黄酮量分别为91.490、115.567、98.528 mg,总黄酮洗脱率分别为85.852%、93.217%、89.051%。以5倍树脂体积的70%乙醇洗脱,流速1.5 m L/min,所得总黄酮回收率为94.09%,纯度为73.77%。结论 AB-8型树脂综合性能最好,其最佳吸附工艺参数为以5倍树脂体积的70%乙醇洗脱,流速1.5 m L/min。Objective To explore the optimum technological parameters of the separation and purification process of total flavones from Malus doumeri( Bois) Chev with macroreticular resins. Methods With the static and dynamic saturated adsorption and elution rate as index,compare the advantages of nonpolar resin D101,weak polarity resin AB-8,medium polarity resin HPD-450 used in isolation and purification of total flavones from Malus doumeri( Bois) Chev,and optimize the adsorption process parameters of selected optimum resin by investigating the total flavones recovery rate. Results The static saturated adsorption of D101,AB-8,HPD-450 were 94. 802,118. 494,98. 317 mg/g dry resin,the static elution rate were 83. 434%,91. 253% and 83. 434% respectively; the amount of total flavones eluted were 91. 490,115. 567,98. 528 mg,elution rate were 85. 852%,93. 217% and 89. 051% respectively. Eluted with five times resin volume of 70% ethanol,flow rate of 1. 5 m L / min,the recovery rate of total flavones was 94. 09%,the purity of which was 73. 77%. Conclusion The comprehensive performance of AB-8 resin was the best,the optimum process parameters were that of eluted with five times resin volume of 70% ethanol and 1. 5 m L / min flow rate.

关 键 词：广山楂总黄酮 大孔吸附树脂 分离纯化 

分 类 号：R284.1[医药卫生—中药学]