心肌局部冷冻对心肌合成一氧化氮的影响  

The effect on synthesis of nitric oxygen in myocardium by local cryoablation

作　　者：赵百亲[1] 章明[2] 朱家光[1] 陈如坤[1] 

机构地区：[1]浙江大学医学院附属第二医院心胸外科 [2]浙江大学医学院解剖学教研室,浙江杭州310009

出　　处：《中国临床解剖学杂志》2003年第5期492-494,共3页Chinese Journal of Clinical Anatomy

基　　金：浙江省自然科学基金资助项目 (397484)

摘　　要：目的 :研究冷冻对心肌合成一氧化氮的影响 ,初步探讨冷冻阻滞心脏传导的机理。方法 :采用液氮对心肌局部冷冻 ,温生理盐水复温 ,冷冻前后测定心肌中一氧化氮及一氧化氮合酶的含量 ,以及冷冻前加L 精氨酸和美兰 ,观察药物的保护及抑制作用。结果 :心肌冷冻后一氧化氮和一氧化氮合酶的含量均下降 ,冷冻前加L 精氨酸对心肌合成一氧化氮有保护作用 ,而冷冻前加美兰对心肌合成一氧化氮有阻滞作用。冷冻后血液中的一氧化氮的含量未见改变。结论 :心肌冷冻后心肌中一氧化氮 (NO )的含量减少 ,而一氧化氮减少 ,会对心肌细胞的传导产生影响。Objective: To study the effect on synthesis of nitric oxygen in myocardium of local cryoablation and method of conduction blocked by cryoablation.Methods: Myocardium was cryoablated locally by a probe cooled to -60℃ and rewarmed by normal salt solution.Nitric oxygen and its synthesis enzyme were measured before and after cryoablation. L-arginine or methylene blue is added before cryoablation,then the effects of these drugs on synthesis nitric oxygen were studied. Result: Nitric oxygen and its synthesis enzyme decreased after cryoablation; L-arginine preserved the nitric oxygen, and methylene blue inhibited the synthesis of nitric oxygen.Nitric oxygen in serum didn't change. Conclusion: Nitric oxygen and its synthesis enzyme decrease after cryoablation and it will effect the conduction in myocardium.

关 键 词：心肌 冷冻 一氧化氮 合成 一氧化氮合酶 心脏传导 

分 类 号：R654[医药卫生—外科学]