色氨酸标记的GCN4单体肽与DNA位点的分子识别  

Molecular Recognition of GCN4 Monomeric Peptide Tryptophane Mutant with Its DNA Target Sites

作　　者：王旭[1] 邓巍[1] 曹傲能[1] 来鲁华[1] 

机构地区：[1]分子动态与稳态结构国家重点实验室

出　　处：《物理化学学报》2003年第9期834-838,共5页Acta Physico-Chimica Sinica

基　　金：国家自然科学基金(20173001;2020331)~~

摘　　要：酵母转录激活因子GCN4调控细胞中氨基酸的生物合成,是典型的含bZIP结构域的DNA结合蛋白.本文合成了天然蛋白GCN4的碱性区(226-252),并在其N末端引入色氨酸残基W,做为单体肽GCN4-W.圆二色(CD)实验表明,突变后的单体肽仍能序列特异性识别DNA结合位点AP-1和ATF/CREB.用荧光滴定方法获得了GCN4-W与DNA位点结合形成复合物的表观解离常数.The transcriptional activator GCN4 plays a key role in the regulation of amino acid anabolism in yeast. It is a DNA binding protein that has typical bZIP domain. We synthesised the basic region (226-252) of natural protein GCN4, and introduced a Trp residue at the N-terminal. CD experimental data show that the synthesised monomer peptide, GCN4-W, can still specifically recognize the DNA target sites AP-1 and ATF/CREB. We also obtained the apparent dissociation constant of the peptide-DNA complex of this monomer recognizing DNA site by fluorescence titration method.

关 键 词：色氨酸 GCN4 单体肽 DNA 位点 分子识别 圆二色 酵母转录激活因子 荧光滴定 解离常数 

分 类 号：Q75[生物学—分子生物学]