冷热刺激对犬血清皮质醇、促肾上腺皮质激素及血液生理指标的影响  

Effect of Cold and Hot Stress on the Concentrations of Cortisol and ACTH in Serum and the Blood Indices of Dogs

作　　者：赵恩军[1] 华修国[1] 张斌[1] 李华威[1] 谢爱纯[1] 

机构地区：[1]上海交通大学农业与生物学院动物科学系,上海201101

出　　处：《畜牧兽医学报》2003年第5期457-460,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：上海市科委攻关项目(990213035)

摘　　要：对体重为4 5±0 5kg的雄性京巴、沙皮和杂种宠物犬,随机分成2组,每组9只,由桡静脉采取自身对照血液后,冷应激组置于0℃的冰水混合物中30min,分别于刺激结束后0h、1h、2h采血;热应激组置于41～42℃的环境中1h,分别于刺激30min和60min时,刺激结束后1h和2h采血。测定血样中的红细胞数、白细胞数、血红蛋白含量、红细胞比容、血沉、红细胞脆性等血液指标,并用放射免疫法测定血清中的皮质醇、促肾上腺皮质激素(ACTH)的浓度。结果表明:在热刺激条件下,各试验期皮质醇和ACTH的浓度均高于各自对照期,但热刺激30min和60min时,与各自对照期相比,皮质醇浓度和ACTH浓度差异均极显著(P<0 01);刺激结束后1h,皮质醇浓度与对照期相比差异仍极显著(P<0 01),ACTH浓度差异显著(P<0 05);刺激结束后2h二者虽都高于对照期,但差异不显著(P>0 05)。冷刺激条件下,各试验期皮质醇和ACTH的浓度均高于各自对照期,其中冷刺激结束时均差异极显著(P<0 01),结束后1h差异显著(P<0 05),结束后2h差异不显著(P>0 05)。冷热刺激对各项血液生理指标也都有或多或少的影响。以上结果显示:上述神经内分泌和血液生理指标的改变与急性冷热应激反应有关,提高了机体对环境变化的适应能力,从而保证生命活动的正常进行。Male Pekingeses,sharpei and crossbreed dogs(45±05 kg)were divided into two groups at random(nine dogs each group),then they were put into the cold environment(0℃)for half an hour or the hot environment(41～42℃)for one hour respectively.At each stage of experiment,we extracted blood from radial veins of the dogs and examined the concentrations of cortisol and ACTH in serum and the blood indices.The results showed that:(1)After the hot stress,the concentrations of cortisol and ACTH increased highly,and increased very strikingly(P<001) at the periods of 30 min and 60 min after hot stress started.Even at the stage of one hour after hot stress the concentration of cortisol was still significantly higher than that in control period(P<001),with the concentration of ACTH being very higher,too(P<005).(2)At the end of cold stress,both of the concentrations of cortisol and ACTH were very notably higher than those in control period(P<001).One hour later after cold stress,the concentrations were significantly higher than those in control period(P<005).At the stage of two hours after cold stress,they were still higher,but not notable(P>005).(3)The cold and hot stress had a fairly notable effect on the blood indices of dogs.The results indicated that the cold and hot stress changed the endocrine and the blood indices of dogs.

关 键 词：冷热刺激 犬 血清皮质醇 促肾上腺皮质激素 血液 生理指标 影响因素 桡静脉 体重 

分 类 号：S829.2[农业科学—畜牧学] S852.21[农业科学—畜牧兽医]