应用Sj23基因重组抗原诊断牛、羊血吸虫病研究  

Diagnosing of Bovine and Sheep Schistosomiasis by Using Sj23 Recombinant Antigen

作　　者：林娇娇[1] 李浩[1] 陆珂[1] 石耀军[1] 傅志强[1] 蔡幼民 

机构地区：[1]中国农业科学院上海家畜寄生虫病研究所 上海动物生物技术研究中心,上海200232

出　　处：《畜牧兽医学报》2003年第5期506-508,共3页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家科技部科研院所社会公益性研究专项基金(2000-181);上海市科技兴农项目资助(00-5-8)。

摘　　要：以血吸虫基因重组抗原LHD Sj23/pGEX(血吸虫23kD抗原大亲水区多肽/pGEX载体表达蛋白)作为抗原,应用ELISA法检测黄、水牛和绵羊血吸虫病。结果对黄、水牛的阳性符合率为94 0%(79/84)和85 5%(53/62),阴性符合率为82 7%(81/98)和95 1%(77/81),对绵羊的阳性符合率为86 04%(111/129),阴性符合率为100%(91/91)。以血吸虫成虫抗原(SWAP)及虫卵抗原(SEA)作为诊断抗原获得的结果差异不明显。对锥虫感染绵羊血清不出现交叉反应。由于基因重组抗原的制备比虫体抗原制备简便、价廉,同时由于基因重组抗原的应用有利于诊断技术的标准化,以基因重组抗原作为血吸虫病诊断抗原具有良好的研究应用前景。The fusion protein LHDSj23/pGEX was employed as diagnosing antigen for detecting bovine and sheep schistosomiasis by ELISA.The result showed that 94.0%(79/84),85.5%(53/62)and 86.04%(111/129)positive coincidence rates,82.7%(81/98),95.1%(77/81)and 100%(91/91)negative coincidence rates were obtained from yellow cattle,water buffaloes and sheep respectively.The similar results were obtained by using SWAP,SEA as diagnosing antigen comparing with that of recombinant LHDSj23/pGEX.No cross reaction was found in 24 sheep Trypanosomosis. The study showing that using Sj23 recombinant antigen as the diagnosis reagent for schistosomiasis will have good potential for applying in the field.

关 键 词：应用 Sj23基因重组抗原 诊断 牛 羊 血吸虫病 阳性符合率 阴性符合率 交叉反应 

分 类 号：S858.23[农业科学—临床兽医学] S858.26[农业科学—兽医学]