使用Lewis肺癌细胞建立昆明种小鼠肺癌模型初探  

Preliminary probing on establishing Lewis lung cancer of the Kunming Mice

作　　者：曹慧慧[1] 张瑞卿[1] 石荣伟[1] 郭晓峰[1] 李俊莲[1] 

机构地区：[1]山西中医学院,山西太原030024

出　　处：《山西中医学院学报》2015年第3期16-18,共3页Journal of Shanxi College of Traditional Chinese Medicine

基　　金：国家自然基金(81173155);国家自然基金(81173381);山西省自然基金(2013011046-9);山西省国际合作项目(2014081049-6)

摘　　要：目的:探讨建立昆明种小鼠Lewis肺癌模型的可行性。方法:将32只雌雄各半的昆明组小鼠随机分为空白组和模型组,将Lewis肺癌细胞悬液按0.2 m L(细胞含量1×107/m L)于模型组16只昆明种小鼠右腋皮下注射,观察其一般状况及成瘤率、肺癌形成率。结果:与空白组相比,模型组小鼠饮食量减少,体重增加缓慢,成瘤率及肺癌形成率均为68.75%。结论:使用Lewis肺癌细胞可以建立昆明种小鼠肺癌模型。Objective:To investigate the feasibility of establishment of Lewis lung cancer model in Kunming mice. Methods:32 Kunming mice,half male and half female,were randomly divided into blank group and model group,each with eight female and eight male. The Lewis lung cancer(LLC)cells suspension was injected into the right armpit of the model group by Subcutaneous with 0.2 m L(1×107/m L). General condition and the tumor generation rate were observed. Result:Compared with the blank group,the mice in model group intake decreased and weight increased slowly. The tumor generation rate and lung cancer generation are 68.75% and 68.75% respectively in model group. Conclusion:The Lewis lung cancer model can be successfully established in the Kunming mice.

关 键 词：LEWIS肺癌 昆明种小鼠 肺癌模型 细胞培养 

分 类 号：R285.5[医药卫生—中药学] R[医药卫生—中医学]