蓝萼香茶菜高效液相指纹图谱研究  

Study on chromatographic fingerprint of Isodon japonica by HPLC

作　　者：杨丽霞[1] 朱晓红[2] 刘必旺[1] 

机构地区：[1]山西中医学院,山西太原030024 [2]解放军第202医院,辽宁沈阳100003

出　　处：《山西中医学院学报》2015年第3期27-29,共3页Journal of Shanxi College of Traditional Chinese Medicine

基　　金：国家"十二五"科技支撑项目(2011BAI07B01;2013BAI05B08)

摘　　要：目的:采用RP-HPLC法建立蓝萼香茶菜药材的指纹图谱。方法:采用Diamonsil钻石C18色谱柱(250 mm×4.6 mm,5μm);流动相为甲醇-水(体积比70∶30),流速为1.0 m L/min,柱温为20℃,检测波长为231 nm。结果:建立了蓝萼香茶菜药材的RP-HPLC指纹图谱,5批蓝萼香茶菜药材指纹图谱R≥0.962 7。结论:采用RP-HPLC指纹图谱检测技术可以有效控制蓝萼香茶菜药材的内在质量。Objective:To study and establish the chromatographic fingerprint of Isodon japonica by RP-HPLC. Methods:The chromatographic conditions were as follows.A Diamonsil C18 column was used and the mobile phase was composed of methanol and water(70∶30). The flow rate was 1.0 m L/min,the column temperature was 20 ℃ and the detection wavelength was set at 231 nm. Results:The chromatographic fingerprint of Isodon japonica was established. The similarity of 5batches of Isodon japonica was not lower than 0.962 7. Conclusion:The quality of Isodon japonica can be controlled effectively by HPLC fingerprint.

关 键 词：蓝萼香茶菜 指纹图谱 RP-HPLC 

分 类 号：R284.1[医药卫生—中药学]