抗栓小肽RWR的毒理学试验研究  

Toxicological assessment of antithrombotic small peptide RWR

作　　者：郑海锋[1] 赵海霞[1] 王少华[1] 杨涛[1] 杨利军[1] 

机构地区：[1]山西医科大学生物化学与分子生物学学科省部共建细胞生理学教育部重点实验室,山西太原030001

出　　处：《中国生化药物杂志》2015年第8期6-10,共5页Chinese Journal of Biochemical Pharmaceutics

基　　金：国家科技部重大新药创制计划(2012ZX09103301-010);山西省自然科学基金(2012011041-5);国家自然科学基金青年基金(81101895);山西省高等学校优秀青年学术带头人支持计划[晋教科(2012)10号]

摘　　要：目的对具有自主知识产权的抗栓小肽RWR进行毒理学研究和安全性评价。方法分别对RWR进行小鼠急性毒性试验、小鼠骨髓细胞微核形成试验、小鼠精子畸形试验和大鼠30d喂养试验。结果急性毒性试验动物经腹腔注射MTD大于120mg/kg;骨髓细胞微核试验、精子畸形试验结果均为阴性;大鼠30 d喂养试验对大鼠体质量、食物利用率、脏器系数、血生化等各项指标均未见不良影响。病理组织学观察肺、肝、脾、肾、心脏均未见明显与注射样品有关的组织学病理改变。结论在本试验条件下,RWR对动物的生长发育、造血功能、肝肾功能、器官组织均无明显毒性作用。Objective To evaluate the toxicological safety of antithrombotic small peptide RWR with independent intellectual property right. Methods Toxicity test in mice, mice bone marrow micronucleus assay, mice sperm abnormality test, and 30-day feeding test in rats were carried out in this study.ResuIts The MTD of acute toxicity test for rats was larger than 120 mg/kg.The results of bone marrow micronucleus assay, mice sperm abnormality test were all negative.Thirty-day feeding test had no adverse effects on body weight, food utility rate, organ coefficient, blood routine index of rats.There was also no histological changes related to RWR in lung, liver, spleen, kidney and heart of rats.ConcIusion RWR had no obvious toxicity on animal growth, hematopoietic function, kidney function and organ tissue and so on.

关 键 词：RWR 毒理学 安全性 

分 类 号：R114[医药卫生—卫生毒理学]