棉花G.LEC1A基因的克隆和鉴定  

Cloning and Identification of G.LEC1A Gene from Cotton(Gossypium barbadense)

作　　者：樊可心 曹爱萍[1] 张文婷[1] 郝小军[2] 崔百明[1] 

机构地区：[1]石河子大学生命科学学院/农业生物技术重点实验室,石河子832003 [2]石河子大学农学院/生产建设兵团绿洲农业生态重点实验室,石河子832003

出　　处：《石河子大学学报（自然科学版）》2015年第5期535-540,共6页Journal of Shihezi University(Natural Science)

基　　金：农业部转基因生物新品种培育科技重大专项(2011ZX08005-002)

摘　　要：为了研究棉花LEC1基因的功能,本研究采用同源克隆方法从海岛棉中克隆棉花LEC1基因(G.LEC1A),构建p CAMG.LEC1A植物表达载体,用农杆菌蘸花法转化拟南LEC1缺失突变体lec1-1,通过干燥筛选方法检验LEC1功能恢复情况。结果表明:G.LEC1A基因的ORF全长为627 bp,编码208个氨基酸,具有LEC1蛋白家族保守的B结构域。组成型表达G.LEC1A可恢复拟南芥lec1-1突变体种子的干燥耐受性。研究结果说明,棉花LEC1A基因是拟南芥LEC1的同功基因。To explore the functions of LEC1 gene in cotton,a homologous gene of LEC1 was cloned from G.barbadense,which was named as G.LEC1 A.The plant expression vectors p CAMG.LEC1 A were constructed,and then it was transformed into Arabidopsis LEC1 defect mutant lec1- 1 by Agrobacterium mediated floral dip method forgene complementation test.The results showed that G.LEC1 A gene contains a 627 bp lengths of open reading frame(ORF) which encodes a polypeptide of 208 amino acid residues with a conserved B domain of LEC1- type proteins.The G.LEC1 A gene was expressed in transgenic Arabidopsis plants and the transgenic plants restored the seeds desiccation tolerance of lec1- 1.These results suggest that G.LEC1 A is a functional homolog of Arabidopsis LEC1.

关 键 词：棉花LEC1基因 载体构建 遗传转化 干燥筛选 

分 类 号：S562[农业科学—作物学] Q943.2[生物学—植物学]