电针对魏一凯二氏大鼠抑郁模型大鼠海马CA1区突触可塑性及5-羟色胺转运体蛋白的影响  

Effect of Electroacupuncture on Synaptic Plasticity and 5-Htt Protein In Hippocampal Ca1 Region of WKY Depression Model Rats

作　　者：韩栩珂 尹平[1] 殷萱 武上雯 徐世芬[1] Han Xuke;Yin Ping;Yin Xuan;Wu Shangwen;Xu Shifen(Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200071, China)

机构地区：[1]上海中医药大学附属市中医医院,上海200071

出　　处：《世界科学技术-中医药现代化》2018年第12期2248-2254,共7页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委青年科学基金项目(81704145):基于5-HT/p11/SMARCA3信号通路以慢病毒沉默p11探讨电针对抑郁大鼠海马突触可塑性的作用机制;负责人:尹平

摘　　要：目的:观察电针对WKY(Wistar Kyoto)抑郁大鼠行为学、海马CA1区突触可塑性、5-HTT蛋白表达的影响。方法:本实验选取10只雄性Wistar大鼠为正常组(Control),30只雄性WKY大鼠随机分成模型组(Model)、电针组(EA)和假针组(Sham EA)。通过糖水偏好实验(SPT)、旷场实验(OFT)和强迫游泳实验(FST)进行行为学测试。采用电生理场电位实验,检测电针对抑郁模型大鼠海马CA1区LTP的作用,即对突触可塑性的影响。并使用Western blot方法检测大鼠海马CA1区5-HTT蛋白表达。结果:Model组糖水偏好比低于正常组(P <0.01),EA组糖水偏好比明显高于Sham EA组(P <0.05)。OFT中,与正常组相比较,Model组中央运动时间,总运动时间,中央运动距离,总运动距离,垂直站立次数均减少,且差异有统计学意义。与Model组相比,电针EA组的中央运动时间和总运动时间明显增加(P <0.05)。FST中,与正常组相比较,Model组大鼠的不动时间明显增加(P <0.05)。与Sham EA组和Model组相比较,EA组的不动时间明显缩短(P <0.05)。Sham EA和Model组LTP均诱导失败,EA治疗后两个脑区均可成功诱导出LTP。Model组兴奋性突触后场电位(Fieldexcitatory postsynaptic potential, fEPSP)斜率明显低于Control组(P <0.001),与Sham EA组相比,EA组fEPSP斜率明显升高(P <0.001)。与Control组相比,Model组5-HTT蛋白的表达显著增高P <0.001)。与Sham EA相比较,EA组5-HTT的表达降低(P <0.001)。结论:电针能够改善WKY大鼠的抑郁样行为,下调海马CA1区5-HTT蛋白的过表达,从而修复受损的海马突触可塑性,这可能是电针治疗抑郁症的机制之一。Objective: To observe the effects of electroacupuncture(EA) on depressive-like behavior, synaptic plasticity and 5-HTT protein expression of hippocampal CA1 region in the WKY model rats. Methods In this experiment, 10 male Wistar rats were selected as the normal group(Control), and 30 male WKY rats were randomly divided into model group,electroacupuncture group(EA) and sham needle group(Sham EA). Behavioral testing was performed by the Sour Water Preference Experiment(SPT), the Open Field Experiment(OFT), and the Forced Swimming Experiment(FST). The electrophysiological field potential experiment was used to detect the effect of electroacupuncture on LTP in the hippocampal CA1 region of rats with depression, which is the effect on synaptic plasticity. The expression of 5-HTT protein in hippocampal CA1 region was detected by Western blot. Results In the SPT, compared to the Control group, the sucrose solution intake was significantly reduced in the Model group(P < 0.01). It was improved after EA treatment compared to Sham EA group(P < 0.05). In the OFT, compared with the Control group, the center time, total move time,center distance, total distance, rearing and grooming incidents were all decreased in the Model group. Compared with the Model group, the center time and total move time in the EA group increased significantly after 3 weeks of treatment(P <0.05). In the FST, duration of immobility was significantly(P < 0.05) longer in the Model group comparing with the Control group. Compared with Sham EA group and Model group, the immobile time of EA group was significantly shorter(P < 0.05). Compared with the Control group, the fEPSP slope of the Model group was significantly shorter(P < 0.001).Compared with the Sham EA group, the fEPSP slope of the EA group increased significantly(P < 0.001). And the LTP of Sham EA and Model groups induced failure in hippocampal CA1 region, the LTP induction was successful after EA treatment. Compared with the Control group, the expression of 5-HTT protein was significantly increa

关 键 词：电针 抑郁症 行为学 突触可塑性 CA1 5-HT 

分 类 号：R245[医药卫生—针灸推拿学] R-332[医药卫生—中医临床基础]