孤雌活化诱导恢复的小鼠减数分裂期间细胞骨架的动态与作用  

The Dynamics and Functions of Cytoskeleton During Meiotic Resumption Induced by Parthenogenetic Activation in Mouse Oocytes

作　　者：朱子玉[1] 文端成[1] 韩之明[1] 雷蕾[1] 刘忠华[1] 寇朝辉[1] 徐营[1] 王敏康[1] 陈大元[1] 

机构地区：[1]中国科学院动物研究所生殖生物学国家重点实验室

出　　处：《生物化学与生物物理进展》2003年第5期819-823,共5页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金资助项目(3 93 60 0 2 8)~~

摘　　要：减数分裂的顺利完成是胞质分裂和核分裂在时间和空间上的协调结果,细胞骨架系统在减数分裂的一系列事件中具有重要的调节作用.实验通过孤雌活化诱导小鼠MⅡ期卵减数分裂恢复,采用激光共聚焦显微术检测了减数分裂期间的微管、微丝和核的动态变化,并通过细胞骨架药物处理,以分析微管和微丝在减数分裂事件中的不同作用.结果显示:纺锤体微管为核的定位、分离和运动所必需;纺锤体从与质膜平行旋转至与质膜垂直是极体排放的前提;微丝是控制纺锤体旋转的关键因素;纺锤体旋转完成后微丝随即解聚,不参与极体的最后排出。The completion of meiosis requires the spatial and temporal coordination of cytokinesis and karyokinesis. Cytoskeleton system is important for regulating a series of events during meiotic maturation. M II stage mouse oocytes were induced to resume meiosis by parthenogenetic activation, and part of them were treated with cytochalasin B and nocodazole, drugs against cytoskeleton. In order to study functions of microtubules and microfilaments in meiosis, the dynamic changes of microtubules, microfilaments and chromosomes were observed by fluorescent confocal microscopy, and the following results were obtained. Meiotic spindle made up of microtubules is essential to location, separation and movement of chromosomes. The spindle rotation from parallel to vertical with respect to plasmalemma is a premise of polar body extrusion. Microfilaments play a crucial role in regulating spindle rotation. After fulfilling the rotation, microfilaments depolymerize immediately, and therefore do not participate in final extruding of polar body. After forming the pronuclei, microfilaments will assemble again, so as to control the migration of pronuclei.

关 键 词：孤雌活化 诱导恢复 小鼠 减数分裂 细胞骨架 

分 类 号：Q245[生物学—细胞生物学] Q952