纳米羟基磷灰石的表征及其对肝癌抑制作用的研究  

Study on Characterizition of Nano-HAP and Its Inhibition on Human Hepatocellular Carcinoma Cell Lines

作　　者：曹献英[1] 李世普[1] 任卫[1] 江昕[1] 王友法[1] 闫玉华[1] 

机构地区：[1]武汉理工大学材料科学与工程学院,武汉430070

出　　处：《硅酸盐通报》2003年第4期21-24,共4页Bulletin of the Chinese Ceramic Society

基　　金：湖北省科技攻关重大项目 ( 2 0 0 2AA10 5A)

摘　　要：利用化学方法辅助超声制备出稳定单分散的羟基磷灰石 (HAP)纳米粒子体系。应用原子力显微镜 (AFM)、粒径电位仪对HAP纳米粒子进行表征 ;采用形态学观察和MTT比色法研究HAP纳米粒子对细胞系Bel- 74 0 2人肝癌细胞的作用 ;从单细胞荧光元素微区分析、超微结构及细胞周期的改变研究其作用机理。实验结果表明 :HAP纳米粒子在体外对Bel- 74 0 2人肝癌细胞具有明显的抑制作用 ,通过进入到癌细胞内 ,阻滞细胞周期的进展 ,使细胞在G1期堆积 ,导致癌细胞胀亡。The stable and single-dispersed HAP nanoparticles were synthesized with chemical method assis ted by ultrasonic treatment.HAP nanoparticles were surveyed by AFM and Zataplus.Their effect on the Bel-7402 human hepatoma cell lines was investigated by the MTT methods and observation of morphology,and the mechanism was studied from changes in cell cycle and ultrastructure and analysis of fluorescence element in micro-area.The result showed that inhibition of HAP nanoparticles on the Bel-7402 human hepatocellular carcinoma cell lines was presented obviously in vitro.HAP nanoparticles entered into cancer cytoplasm,and cells'proliferation was stopped at G 1 phase of cell cycle, thus,cancer cells died directly.

关 键 词：纳米羟基磷灰石 表征 肝癌 抑制作用 纳米粒子 化学方法辅助超声 制备 

分 类 号：TQ462[化学工程—制药化工] R979.1[医药卫生—药品]